摘要
研究了溶氧浓度对产甘油假丝酵母分批发酵生产甘油过程的影响。实验结果表明:当溶氧浓度控制在30%时,C.glycerinogenes的甘油产量、得率和产率达到最高,分别为120.7 g/L、0.575g/g和1.69g/(L.h),而糖酵解代谢副产物形成最少。当溶氧浓度为10%时,发酵过程呈现出"巴斯德效应"的特征,生成的酵解代谢副产物维持在较高水平。在快速生长阶段,随着溶氧从10%增加到60%,细胞呼吸类型表现为从厌氧呼吸向好氧呼吸转变,酵解代谢副产物依次减少。在生长稳定期,控制的溶氧浓度越高,酵解代谢副产物乙醇、乙酸等的生成就越少。分别选用Logistic方程、Luedeking-Piret方程和Luedeking-Piret-like方程,能较好地模拟细胞生长、甘油合成和葡萄糖消耗的动力学过程,并用实验得到了验证。
The influence of dissolved oxygen （DO）concentration in the range of 10% - 60% （of air saturation） on glycerol production by Candida glycerinogenes in batch fermentation was investigated by using the chemically defined medium. The results showed that it was optimal for glycerol production by C. glycerinogenes at DO concentration of 30% ,and the glycerol concentration,yield and productivity reached 120.7 g/L,0.575 gig and 1.69 g/（ L · h）, respectively, while the glycolytic by-products formation was little. At DO concentration of 10% ,the “Pasteur effect” was observed during the fermentation, so the glycolytic by-products were at a high level During the rapid growth phase, the cells could gradually transit their anaerobic fermentation to aerobic metabolism and the formation of glycolytic by-products was reduced when the DO concentration increased from 10% to 60%. However,the oxygen consumption of cells decreased sharply during the steady growth stage. With an increase of DO concentration, the amount of ethanol and acetic acid was also gradually decreased. equation, Luedeking -Piret equation kinetics of cell growth, glycerol concentrations, which were validated Logistic and Luedeking-Piret-Like equation was successfully used to describe the production and glucose consumption, respectively, under diverse DO by the experiments.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第5期65-70,共6页
China Biotechnology
关键词
溶氧
产甘油假丝酵母
甘油合成
动力学
Dissolved oxygen Candida glycerinogenes Glycerol production Kinetics
