摘要
研究了产甘油假丝酵母细胞回用生产甘油的反复分批发酵。实验结果表明：第一级发酵培养基KH2PO4浓度为0．4g·L^-1，回用培养基的最适KH2PO4浓度为0．1g·L^-1，当上一批次发酵液中葡萄糖浓度降至10g·L^-1以下时，酵母细胞不经洗涤即可回用；经过15个批次的反复分批发酵过程，甘油的平均产量、平均得率和平均生产能力分别达到138．69g·L^-1、60．17％和2．31g·L^-1·h^-1，分别比第一级发酵结果增加了15．74％、15．48％和39．16％；但回用至第15次时，菌体出现严重衰退，因此回用周期以12～14次为宜。
Glycerol production by reusing free Candida glycerinogenes cells in repeated batch fermentation was studied. The results showed that the suitable concentration of KH2PO4 in the re-inoculation medium was 0. 1 g · L^-1 , lower than KH2PO4 of 0.4 g 2·L^-1 in the first fermentation medium. When the residual glucose concentration decreased to 10 g · L^-1 , yeast cells from sterile centrifugation with the activity high enough for the next cycle could be directly recycled for the next batch fermentation. After 15 cycles of reinoculation, average glycerol concentration, yield and productivity were 138.69 g ·L^-1 , 60.17% and 2.31 g ·L^-1·h^-1, with an increase of 15.74%, 15.48% and 39.16%, respectively, compared with these in the first batch fermentation. Although yeast cells decayed severely in the fifteenth cycle, 12-14 cycles of repeated batch fermentation were possible.
出处
《化工学报》
EI
CAS
CSCD
北大核心
2007年第1期195-199,共5页
CIESC Journal
基金
国家自然科学基金项目(30570142)
江苏省青年科技创新人才(学术带头人)基金项目(BK2006504)~~
关键词
产甘油假丝酵母
细胞回用
甘油
反复分批发酵
Candida glycerinogenes
recycle of cells glycerol
repeated batch fermentation
