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产甘油假丝酵母反复分批发酵法生产甘油

Glycerol production by repeated batch fermentation of Candida glycerinogenes
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摘要 Glycerol production by repeated batch fermentation of Candida glycerinogenes was studied by using not only synthetic medium but also complex medium.The results showed that the increase rates of glycerol production,glycerol yield on initial glucose and glycerol productivity were beyond 15.0%,15.0% and 37.0% when yeast cells are recycled 13 times in low phosphate synthetic medium,9 times in low phosphate complex medium and 14 times in low phosphate complex medium with supplemented trace elements,respectively.The number of recycling in low phosphate complex medium was fewer than that in low phosphate synthetic medium.It is because low phosphate synthetic medium only limited phosphate concentration,while low phosphate complex medium limited both trace elements and phosphate.The shortage of trace elements solution influenced cell growth and glycerol synthesis,and reduced recycling number of yeast cells in low phosphate complex medium.The limitation of phosphate concentration in repeated batch fermentation medium was helpful to promoting the ability of glycerol synthesis of C.glycerinogenes. Glycerol production by repeated batch fermentation of Candida glycerinogenes was studied by using not only synthetic medium but also complex medium. The results showed that the increase rates of glycerol production, glycerol yield on initial glucose and glycerol productivity were beyond 15.0 %, 15.0 % and 37.0% when yeast cells are recycled 13 times in low phosphate synthetic medium, 9 times in low phosphate complex medium and 14 times in low phosphate complex medium with supplemented trace elements, respectively. The number of recycling in low phosphate complex medium was fewer than that in low phosphate synthetic medium. It is because low phosphate synthetic medium only limited phosphate concentration, while low phosphate complex medium limited both trace elements and phosphate. The shortage of trace elements solution influenced cell growth and glycerol synthesis, and reduced recycling number of yeast cells in low phosphate complex medium. The limitation of phosphate concentration in repeated batch fermentation medium was helpful to promoting the ability of glycerol synthesis of C. gl ycerinogenes.
出处 《化工学报》 EI CAS CSCD 北大核心 2009年第6期1529-1533,共5页 CIESC Journal
基金 湖南省自然科学基金项目(07JJ6031)~~
关键词 产甘油假丝酵母 反复分批发酵法 甘油生产 限磷 Candida gl ycerinogenes phosphorus limitation repeated batch fermentation glycerol production
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参考文献12

  • 1Mohammad J, Taherzadeh, Lennart A. Strategies for enhancing fermentative production of glycerol: a review. EnzymeMicrob. Technol., 2002, 31: 53-66. 被引量:1
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二级参考文献12

  • 1谢涛,方慧英,诸葛健.磷源对产甘油假丝酵母发酵生产甘油的影响及动力学分析[J].化工学报,2005,56(12):2404-2409. 被引量:12
  • 2Mohammad J,Taherzadeh,Lennart A,Gunnar L.Strategies for enhancing fermentative production of glycerol:a review.Enzyme Microb.Technol.,2002,31:53-66 被引量:1
  • 3Zhuge J,Fang H Y,Wang Z X.Glycerol production by a novel osmotolerant yeast Candida glycerinogenes.Appl.Microbiol.Biotechnol.,2001,55:686-692 被引量:1
  • 4Wang Z X,Zhuge J,Prior B A.Glycerol producing by microbial fermentation:a review.Biotechnol.Adv.,2001,19:210-223 被引量:1
  • 5Liu Y Q,Liu D H.Kinetic study on glycerol production by repeated batch fermentation using free Candida krusei.Process Biochemistry,2004,39:1507-1510 被引量:1
  • 6Bemito G G,Ozores M,Pena M.Continuous glycerol production in a packed-bed bioreactor with immobilized cells of Saccharomyces cerevisiae.Bioresource Technology,1994,49:209-212 被引量:1
  • 7Mostafa N A,Magdy Y H.Utilization of molasses and akalona hydrolyzate for continuous glycerol production in a packed-bed bioreactor.Energy Convers.Mgmt.,1998,39:671-677 被引量:1
  • 8Bisping B,Rehm H J.Production of glycerol by immobilized Pichia farinosa.Appl.Microbiol.Biotechnol.,1990,32:380-383 被引量:1
  • 9Vijaikishore P,Karanth N G.Glycerol production by immobilized cells of Pichia farinosa.Biotechnology Letters,1986,8 (4):257-260 被引量:1
  • 10Lambert M,Neish A C.Rapic method for estimation of glycerol in fermentation solutions.Can.J.Res.,1956,28:83-89 被引量:1

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