摘要
假单胞菌(Pseudomonas sp.)TS1138在含有DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-2-amino-Δ2-thiazoline-4-car-boxylic acid,DL-ATC)的环境中能够转化DL-ATC生成L-半胱氨酸的酶系。通过固定不同溶氧浓度,探索溶氧对假单胞菌TS1138发酵产酶的影响。摇瓶实验结果表明:溶氧浓度过低,菌体生长缓慢,细胞产酶能力较低;溶氧浓度过高,菌体生长速度快,但不利于细胞产酶。7L罐发酵实验结果表明:在产酶中后期,应根据溶氧浓度变化来调节搅拌转速或空气流量,使溶氧浓度控制在30%以上。
Pseudomonas sp.TS1138 has potential to produce L-cysteine synthetase through asymmetrical hydrolysis of DL-2-amino-Δ2-thiazoline-4-carboxylic acid（DL-ATC）.The effect of dissolved oxygen level on the production of L-cysteine synthetase was investigated in shake flasks or 7 L bioreactor.Results indicated that the cell growth and the production of L-cysteine synthetase were inhibited at low dissolved oxygen level.Although cell growth was improved at the high dissolved oxygen level,the inhibition against production of L-cysteine synthetase was still observed in shake flasks.In 7 L bioreactor,dissolved oxygen concentration controlled at more than 30% was helpful for improving the cell growth and the production of L-cysteine synthetase through regulating agitation rate and air flow rate during the middle and late stage.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第3期182-184,共3页
Food Science
基金
天津市科委应用基础研究重点基金项目(05YFJZJC00901)
关键词
L-半胱氨酸
溶氧
假单胞菌
L-cysteine dissolved oxygen Pseudomonas sp.
