摘要
目的筛选适合灰树花产海藻糖的母种培养基配方。方法通过四种不同固体培养基培养灰树花菌种,比较其生长状况,并进行液体摇瓶培养,采用蒽酮—硫酸法测定海藻糖产量。结果在马铃薯板栗壳培养基上生长的灰树花长势强,颜色亮白,菌丝浓密,生长速度快(长速:7.5 mm/d,满管时间:10.8 d);此菌种在液体发酵中生物量和海藻糖产量也很高,分别为17.6 g/L和3.4 g/L。结论马铃薯板栗壳培养基是一种优良的灰树花母种培养基,有利于提高液体发酵中海藻糖的产量。
Objective： To select the mother medium composition of Grifola frondosa for the production of trehalose. Methods： Grifola frondosa strains were cultured to compare their growth by four different solid media, and then cultured by liquid culture flask. Trehalose was determinated by anthrone-sulphufie acid method. Results： Grifold frondosa growing on the potato chestnut shell medium was strong, thick, white and grew quickily （ growth ratio 7.5per day, 10.8days to fill out the test tube）, and the yield of mycelium dry weight was 17.6 g/L and trehalose was 3.41 g/L. Conclusion： Potato chestnut shell medium is a good mother medium of Grifola frondosa, conducive to improving the yield of trehalose from liquid fermentation medium.
出处
《泰山医学院学报》
CAS
2008年第12期952-954,共3页
Journal of Taishan Medical College
基金
泰山医学院青年科学基金项目(2007-2008)
关键词
灰树花
海藻糖
马铃薯板栗壳培养基
发酵培养基
蒽酮—硫酸法
Grifola frondosa
trchalose
potato chestnut shell medium
fermentation medium
anthrone-sulphuric acid method
