摘要
【目的】研究血小板衍化生长因子-B（PDGF-B）和胰岛素样生长因子-1（IGF-＊1）单侧大鼠对局灶性脑缺血／再灌注损伤影响的可能机制。【方法】建立SD大鼠局灶性脑缺血／再灌注模型，按实验随机分组，用TTC染色法测量各组脑梗死体积，用原位末端标记技术TUNEL法对各组大鼠脑缺血中心及周围区神经细胞的凋亡进行检测。【结果】①PDGF—B，IGF-1治疗组有明显的剂量效应关系（P〈0．05或P〈0．01）；②TTC染色及凋亡检测结果显示，与缺血／再灌注组相比，PDGF—B，IGF-1及PDGF-B＋IGF-1治疗组脑梗死体积明显减小（P〈0．01），其中PDGF-B＋IGF—1治疗组最为明显（P〈0．05）。[结论]PDGF-B和IGF1可明显减小脑梗死体积，减少凋亡细胞数，二者联合用药可使疗效更为显著。
[Objective]To establish the damage model of focal cerebral ischamic/reperfusion at one side of the brain in rats and to study the possible mechanism of platelet-derived growth factor-B（PDGF-B）and insulin- like growth factor-1 （IGF-1） for focal cerebral isehamie/reperfusion damage. [Methods]Focal cerebral ischam- ie/reperfusion model in rats was established and divided randomly according to the different observation time. The volume of cerebral infarction in each group was detected by TTC stain. TUNELbased immunoflourescent technique was used to observe apoptosis of neuronal cells at the central and around the focal cerebral isehemia. [Results]（1）PDGF-B, IGF-1 and PDGF B＋IGF 1 treatment groups had obvious relationship between dosage and effective（ P 〈0.05 or P 〈0.01 ）. （2）The result of TTC stain and TUNEL detection showed that compared with ischemia group, the volume of cerebral infarction and the TUNEL positive cells in PDGF-B, IGF-1 and PDGF-B＋IGF-1 treatment group decreased significantly respectively according to different observation time （ P 〈0.01）, especially in PDGF-B＋IGF-1 treatment group （ P 〈0.05）. [Conclusion]PDGF-B or/and IGF 1 can reduce the volume of cerebral infarction and the number of neuronal cells apoptosis greatly. And the combination of them is more effective. But the mechanism should be proved later.
出处
《医学临床研究》
CAS
2009年第3期390-394,共5页
Journal of Clinical Research
关键词
脑缺血
再灌注损伤
血小板源生长因子
胰岛素样生长因子Ⅰ
cerebral ischemia
reperfusion injury
platelet-derived growth factor
insulin-likegrowth factor Ⅰ
