摘要
目的:探讨外源性降钙素基因相关肽(CGRP)和神经生长因子(NGF)对短暂性脑缺血后再灌注大鼠纹皮质c-fosmRNA及蛋白表达的影响。方法:在脑缺血后再灌注模型上,应用原位杂交和免疫组织化学结合显微图像分析方法检测c-fosmRNA及蛋白表达。结果:缺血组大鼠纹皮质较假手术组c-fosmRNA和蛋白表达非常显著增加;CGRP组和NGF组c-fosmRNA和蛋白表达分别显著弱于缺血组;CGRP和NGF合用组c-fosmRNA和蛋白表达非常明显弱于缺血组、CGRP组和NGF组。结论:CGRP和NGF分别抑制脑缺血后再灌注大鼠纹皮质c-fosmRNA及蛋白表达,联合应用则能显著抑制脑缺血后再灌注大鼠纹皮质cfosmRNA及蛋白表达,二者对保护缺血神经元可能有协同作用。
Objective: To study the effect of calcitonin gene related peptide (CGRP) and nerve growth factor (NGF) on the expression of c-fos mRNA and protein in striate cortex of rats with transient cerebral ischemia/reperfusion. Methods: In situ hybridization, immunohistochemistry and micro image analysis were used to observe the expression of c-fos mRNA and its protein in the striate cortex of rats with cerebral ischemia. Results: The expression of c-fos mRNA and protein was higher in ischemia/reperfusion(I/R) group compared with sham group (P<0.01), and was lower in NGF group and in CGRP group compared with I/R group (P<0.05), and markedly lower in NGF&CGRP group than in I/R group (P<0.01) and in NGF group and CGRP group respectively (P<0.05). Conclusion: CGRP and NGF downregulate the expression of c-fos mRNA and its protein, and combined use of NGF & CGRP can markedly downregulate the expression of c-fos mRNA and protein in striate cortex of cerebral ischemia rats, which indicates that CGRP and NGF might have coordinately protective effects on the ischemia neurons in striate cortex.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2005年第3期301-303,共3页
Chinese Journal of Anatomy
基金
辽宁省自然科学基金(619019)
