摘要
采用“搓网”方法将８种９６例不同的恶性肿瘤手术切除标本制成细胞膜完整的单细胞悬液，并从６例癌性腹水中提取富含癌细胞的悬液。用流式细胞免疫学方法检测癌细胞（ＭＤＲ１）表达产物Ｐ１７０含量。经上述方法制成悬液中的癌细胞丰富、荧光显微镜下观察胞膜完整，Ｐ１７０阳性细胞抗体荧光位于胞膜上。本文认为用流式细胞免疫学技术检测恶性实体瘤细胞ＭＤＲ１表达产物Ｐ１７０含量简便易行，不仅可以了解肿瘤细胞始基的药物敏感性，还可在化疗过程中动态监测药物敏感性的变化。
To explore the feasibility of FCM in studying the multidrug resistant gene (MDR1) expression in malignant solid tumors,singlecell suspension with intact cellular membrane taken from 96 operative specimens of 8 different kinds of malignant solid tumor were prepared using rubbingnet technique.Autologous tumor cell (ATC) suspension was also made from 6 ascites samples of advanced ovarian carcinomas. The suspensions were assayed for their MDR1 product P170 (Pgp) contents by FCM immunology. It was found that the suspensions were rich in single neoplastic cells with intact cell membrane. The labelled fluorescence of P170positive cells spotted the membrane. This exemplifies that this technic is highly feasible in studying MDR1 expression of malignant solid tumors and is helpful to perceive inherent nature of multidrug resistance.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
1998年第7期489-491,共3页
Chinese Journal of Clinical Oncology
