摘要
目的建立葛根素的电化学含量测定方法。方法采用库仑阵列检测器研究葛根素的电化学行为及含量测定方法。结果在pH4.32磷酸盐缓冲液中,葛根素在0.16～162ng内与峰面积呈良好线性关系,回归方程为A=1444.7C+88.635,相关系数r=0.9998,平均回收率为96.88%,RSD为1.75%。结论电化学法操作简便,准确,灵敏度高,可用于葛根素的含量测定以及生物样品的测定。
Objective To set up the electrochemical determination method for puerarin in Radix Puerariae. Methods The electrochemical behavior and content determination of puerarin were investigated by using coulomb array detector. Results In phosphate buffer with PH 4.32, puerarin showed good linearity in the range of 0. 16 ng - 162 ng. The regression equation was A = 1444.7C ＋ 88. 635, r = 0. 9998 （ n = 7）, the average recovery was 96.88 %, and RSD was 0. 08 %. Conclusion This method is simple and rapid, and the result is accurate and reliable. It can be used for the determination of puerarin content and biological samples.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2009年第2期140-142,共3页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
广东省科技攻关项目(2005B33001037)
