摘要
目的 建立以高效液相色谱法测定葛根素注射液中葛根素含量的方法。方法 色谱柱为十八烷基键合硅胶柱，流动相：甲醇-1％冰乙酸（1：3），流速：1．2mL／min，检测波长：250nm。结果 在0．32～1．60ug检测范围之间，对照品微克数与峰面积呈良好的线性关系，回归方程Y=2E＋06X＋33911、r=0．9999。平均回收率为99．82％，RSD=0．98％。结论 本方法操作简便、快速、准确，可用于葛根素注射液的质量控制方法。
Objective To establish the determination method of puerarin in puerarin injection. Methods HPLC was used on ODS C18 column , methanol-1%glacial acetic acid （1：3） was used as a mobile phase .The detection wave-length was at 250nm . Results The detection range was 0.32～1.60ug. There was a good linear relationship between consistence and apex-area. The regress equation was Y = 2E＋ 06X＋33911, r=0. 999 9. The recovery of the added sample was 99.82%,and RSD：0. 98 %. Conclusion This method was simple and reliable, and could be used for the control quality of puerarin injection.
出处
《现代中药研究与实践》
CAS
2006年第4期46-47,共2页
Research and Practice on Chinese Medicines
