摘要
目的：建立木香中木香烃内酯含量测定的HPLC法。方法以十八烷基键合硅胶为填充剂，流动相为甲醇—乙腈—水（65∶5∶30），检测波长225 nm，流速1．0 mL·min^－1。结果木香烃内酯在12～120 mg·L^－1范围内线性关系良好，回归方程为Y＝2．16×104X＋92064．7（r＝0．9995），平均加样回收率为98．90％，RSD为1．53％。结论 HPLC法简单易行、准确度高，可用于木香的质量控制，不同产地的木香中木香烃内酯含量差别较大。
Objective To establish an HPLC method for determining costunolide in Aucklandia.Method With eighteen alkyl silane bonded silica as the filler,and methyl alcohol-acetonitrile-water （65∶5∶30）as the mobile phase,the detective wavelength was set at 225 nm and the flow rate was 1.0 mL·min-1.Results The costunolide shows linear correlation at 12~120 mg·L-1（equation was Y=2.16 ×104X＋92 064.7（r=0.999 5）.The average recovery of costunolide was 98.90% and RSD was 1.53%.Conclusions The method developed is simple and accurate,which can be used for quality control of the Radix Aucklandia.The content of costunolide was different in the Radix Aucklandia from different sources.
出处
《安徽医药》
CAS
2015年第5期866-868,共3页
Anhui Medical and Pharmaceutical Journal
