摘要
目的：建立血浆中葛根素的高效液相色谱测定方法，为含葛根素中药复方体内代谢研究提供基础。方法：血浆用０．５８ｍｏｌ·Ｌ－１高氯酸沉淀蛋白质，采用ＳｈｉｍｐａｃｋＣＬＣＯＤＳ柱（５μ，１５０ｍｍ×４．６ｍｍ），预柱为ＹＷＧＣ１８Ｈ３７，流动相为甲醇５．６２５ｍｏｌ·Ｌ－１醋酸水（２５∶３∶７２），流速为１．００ｍｌ·ｍｉｎ－１，检测波长为２４７ｎｍ。结果：该法回收率９１．０％，方法重现性ＲＳＤ＝０．９３％（ｎ＝６）。结论：本文建立的高效液相色谱测定方法为动物体内葛根素的测定提供了一个可靠的方法。
OBJECTIVE:To establish the determining method of puerarin in plasma with high performance liquid chromatography.METHOD:The 0.58 mol·L -1 perchloric acid was used to precipitate the protein.Shimpack CLC ODS column(5 μ,150 mm×4.6 mm) was used as analytical column.YWG C 18 H 37 was used as pre column.The mobile phase consisted of methanol 5.625 mol·L -1 acetic acid water(25∶3∶72).The rate of mobile phase was 1.00 ml.RESULTS:The recovery of this method was 91.0%,and the repeatability was RSD=0.93%(n=6).CONCLUSION:The method could be used to determine puerarin in vivo.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1997年第2期104-106,共3页
Chinese Pharmaceutical Journal
