摘要
目的采用高效液相-库仑阵列电化学法（HPLC-ECD）同时测定青天葵中鼠李柠檬素和鼠李秦素含量,并对不同培养方法及不同生长阶段的青天葵进行测定分析。方法采用Agilent HC-C18色谱柱（150 mm×4.6mm,5μm）,以甲醇∶乙腈（体积比2∶1）混合后加5%20 mmol·L^-1Na H2PO4（A）-20 mmol·L^-1Na H2PO4水溶液,用H3PO4调p H至2.50（B）为流动相,A∶B=48∶52等度洗脱,流速：1 m L·min^-1,进样量15μL,柱温：35℃,检测电压：50,150 m V。结果鼠李柠檬素和鼠李秦素的线性范围分别为0.2-50.1μg·m L^-1（r=0.9999）和0.15-36.4μg·m L^-1（r=0.9999）,平均回收率（n=9）分别为97.98%（RSD=1.50%）和97.64%（RSD=2.49%）。结论 HPLC-ECD法测定青天葵中鼠李柠檬素、鼠李秦素操作简便、准确,灵敏度高,不同培养阶段药材含有的鼠李柠檬素、鼠李秦的含量有一定差异,可为青天葵质量标准制定及提高提供依据。
Objective To establish a method for simultaneous determination of rhamnocitrin and rhamnazin in Folium Nerviliae Fordii（FNF）with high performance liquid chromatography-electrochemical detection（HPLC-ECD）,and to assay FNF cultivated with different methods and FNF at different growth stages. Methods The analytical method was carried out on an Agilent HC-C18column（150 mm ×4.6 mm, 5 μm） with a mobile phase of methanol-acetonitrile（volume ratio being 2∶1）mixed with 5 % of 20 mmol·L^-1Na H2PO4（A）-20 mmol·L^-1Na H2PO4 aqueous solution with p H being 2.50 adjusted by H3PO4（B）,The ratio A to B was 48∶52（A∶B）, flow rate was 1.0 ml·min^-1. The detection voltage was set at 50, 150 m V, column temperature was 35 ℃, and the injection volume was 15 μL.Results The calibration curves were linear within the range of 0.2~50.1μg·m L-1for rhamnocitrin（r=0.9999） and0. 15~36. 4 μg·m L-1for rhamnazin（r=0.9999）. The average recoveries（n=9） were 97.98 % for rhamnocitrin（RSD=1.50 %）and 97.64 % for rhamnazin（RSD=2.49 %）.Conclusion The contents of rhamnocitrin and rhamnazin in FNF vary in different growth stages. Electrochemical detection is convenient,accurate and sensitive,and it can be used for the upgrading of quality standard of FNF.
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2014年第6期733-736,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
中柬第三方检测平台建设项目-2012年广州市白云区国际科技合作计划项目(2012-KQ-23)
关键词
青天葵
鼠李柠檬素
鼠李秦素
高效液相-库仑阵列电化学法
Folium Nerviliae Fordii
Rhamnocitrin
Rhamnazin
High performance liquid chromatography-electrochemical detection
