摘要
目的:建立食品中苯甲酸、山梨酸、糖精钠、安塞蜜、咖啡因、脱氢乙酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯和对羟基苯甲酸丁酯10种添加剂的高效液相色谱测定方法。方法:采用高效液相色谱法,利用C18色谱柱(250×460 mm i.d.,5μm),以0.05 mol/L磷酸二氢铵(用磷酸调pH=4.0)溶液和乙腈为淋洗液,流速1.0 ml/min,通过改变流动相比例进行梯度洗脱,采用紫外波长230 nm和254 nm进行检测,外标法定量。结果:10种添加剂在相应的试验浓度范围内均具有良好的线性关系,其相关系数均大于0.9990,最低检出限在0.04～1.0 mg/L范围,平均回收率在90.0%～105.0%之间,RSD均小于5.0%。结论:该法具有简单、准确、灵敏等优点,适合同时检测食品中10种添加剂含量。
Objective：To develop an analytical method for the determination of additives including benzoic acid,sorbic acid,saccharin sodium,acesulfame-K,caffeine,dehydroacetic acid,methyl p-hydroxybenzoate,ethyl p-hydroxybenzoate,propyl p-hydroxybenzoate and butyl p-hydro xybenzoate in food by HPLC.Methods：The 10 additives in food were separated on C18 column（250×460 mm i.d.,5 μm）by using 0.05 mol/L ammonium phosphate monobasic（pH was 4.0 adjusted by phosphoric acid） and acetonitrile as mobile phase at a flow rate of 1.0 ml/min.The way of gradient elution was adapted.Detection was performed on an ultraviolet detector at λ=230 nm and 254 nm and quantification was calculated by an external standard method.Results：The linearity of additives each in the tested concentration range was good and the correlation coefficients were all more than 0.9990.The limits of quantification were found to be 0.04~1.0 mg/L for the target compounds.The extraction recoveries were between 90.0%~105% and the RSD all less than 5.0%.Conclusion：This method was found to be simple,sensitive and accurate for the determination of the additives in food and it can be satisfactorily applied to the routine analysis.
出处
《中国卫生检验杂志》
CAS
2009年第1期9-11,共3页
Chinese Journal of Health Laboratory Technology
