摘要
目的建立化妆品中苯甲醇、山梨酸、苯甲酸、对羟基苯甲酸甲酯、对羟基苯甲酸乙酯、对羟基苯甲酸丙酯、对羟基苯甲酸丁酯等7种防腐剂的同时测定方法。方法样品经甲醇溶解、超声提取、离心,采用大口径非极性毛细管色谱柱DB-1(15m×0.53mm×0.5μm)气相色谱法进行定性和定量分析,并探讨最佳实验条件。结果该方法的线性范围为苯甲醇100～500μg/ml;山梨酸200～600μg/ml;苯甲酸200～600μg/ml;对羟基苯甲酸酯类均为25～500μg/ml,其相关系数在0.9983～0.9999之间;方法最低检出限分别为5.5、57.1、29.6、6.2、5.5、6.2、6.2ng/ml;样品加标回收率为91.6%～102.7%,相对标准偏差为2.2%～10.2%(n=3)。结论该方法简易可行,并能较好地分离上述7种化合物,具有分析时间短、抗干扰性好、定量准确等优点。
Objective To establish a method for determination of 7 preservatives, benzyl alechol, sorbic acid, benzoic; acid, methyl-paraben, ethyl-paraben, propyl-paraben, butyl-paraben, in cosmetics in one time by gas chromatography. Methods The cosmetic sample was dissolved by methanol, extracted by ultrasonic and separated by packed column of DB1, the optimized experimental condition was explored also. Results The concentrations of 7 preservatives showed a positive correlation with the peak heights at the following ranges, 100-500 μg/ml for benzyl alechol, 200-600 μg/ml for sorbic acid, 25-500 μg/ml for benzoic acid, methyl-paraben, ethyl-paraben, propyl-paraben and butyl-paraben, the correlative coefficients were 0.998 3-0.999 9. The detection limits were 5.5, 57.1, 29.6, 6.2, 5.5, 6.2, 6.2 ng/ml respectively. The recovery rates were 91.6%-102.7%. RSDs were 2.2%-10.2%. Conduslon The present method can perfectly separate the seven preservatives in cosmetics mentioned above with some advantages, such as simple, accurate, sensitive and quick.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2006年第2期171-173,共3页
Journal of Environment and Health
关键词
色谱法
气相
化妆品
防腐剂
Chromatography, gas
Cosmetics
Preservative
