摘要
目的建立反相高效液相色谱（RP—HPLC）法测定苦丁茶含片中槲皮素和山柰素的含量。方法以Nova-Pak C18（3．9mm×300mm，4．0μm）为色谱柱；甲醇-0．4％磷酸溶液（50：50）为流动相；检测波长360nm；流速0．7ml·min^-1；柱温40℃。结果槲皮素的进样量在0．01299～0．6496μg范围内，山柰素的进样量在0．01334—0．6672μg范围内与峰面积呈良好的线性关系。平均加样回收率分别为97．03％和97．34％，RSD分别为1．16％（n=6）及1．42％（n=6）。结论该方法简便、快速、准确，可作为苦丁茶含片中槲皮素和山柰素的含量测定方法。
Objective To establish an RP - HPLC method for determination of the content of quertetin and kaempferol in Kudindcha tablets. Methods The Nova -Pak C18 column （3.9 mm× 300 mm,4. 0μm） was used. The mobile phase was methanol - 0.4% H3 PO4 （50： 50）, the detection wavelength was 360nm, the flow rate was 0. 7 ml·min^-1, the temperature was 40℃. Results The linear range of quertetin was 0.012 99 ～0. 649 6μg , the linear range of kaempferol was 0.013 34 ～0.667 2μg. The average recovery was 97.03% and 97.34% ,RSD was 1.16% （n = 6） and 1.42% （n = 6）. Conclusion This method is simple, rapid and accurate. It can be used for determination of the content of quertetin and kaempferol in Kudindcha tablets.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第1期119-120,共2页
Lishizhen Medicine and Materia Medica Research
基金
广西科学研究与技术开发计划项目(No.0330001-1C)
