摘要
目的采用高效液相色谱法测定罗布麻叶中槲皮素和山柰素含量。方法采用SUPELCO色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.4%磷酸溶液(50∶50),检测波长360 nm,柱温30℃。结果槲皮素、山柰素的进样量分别在0.101 4～0.709 8μg(r=0.999 6)、0.021 6～0.216 08μg(r=0.999 9)线性关系良好。平均回收率分别为98.40%、98.34%,RSD分别为0.98%、0.97%(n=5)。结论本方法操作简便,结果可靠、准确,能有效地评价罗布麻叶的质量。
Objective To determine quercetin quercetin and kaempferol in Apocynum venetum L.by HPLC.Methods HPLC method was used.The separation was performed on a SUPELCO C18 column（4.6 mm×150 mm,5 μm）.The mobile phase was methanol-0.4%phosphoric acid（50∶50）.The detection wavelength was set at 360 nm.The column temperature was 30 ℃.Results The linear range of quencetin and kaempferol was 0.101 4-0.709 8 μg（r =0.999 6） and 0.021 6-0.216 08 μg（r =0.999 9） respectively.The average recovery was 98.40%（RSD=0.98%,n =5） and 98.34%（RSD=0.97%,n =5） respectively.Conclusion The method is simple,reliable,accurate,and can be applied to the quality control of Apocynum venetum L.
出处
《中国中医药信息杂志》
CAS
CSCD
2013年第1期51-53,共3页
Chinese Journal of Information on Traditional Chinese Medicine
