摘要
目的:对壮药茉莉花进行定性定量研究,制定茉莉花的质量标准。方法:采用性状、显微和薄层色谱法(TLC)对茉莉花进行定性鉴别,参照中国药典方法进行检查和浸出物项目测定,采用高效液相色谱法(HPLC)测定茉莉花中槲皮素和山柰素含量。结果:各鉴别方法专属性强,槲皮素和山柰素分别在进样量为0.4008~3.2064μg和0.0403~3.2256μg范围内与高效液相色谱峰面积呈良好线性关系。结论:建立的方法简便准确,重复性好,可有效控制茉莉花药材的质量。
Objective: To study the qualitative and quantitative determination of Zhuang medicine Jasmini Sambacis Flos and es-tablish its quality control standard. Methods: Macroscopic, microscopic and TLC identification were adopted to carry out the qualitative identification, the mensuration of inspection items and extractum were according to the Chinese Pharmacopoeia. The contents of querce-tin and kaempferide in Jasmini Sambacis Flos were determined by HPLC. Results: The qualitative identification methods had strong specificity. In HPLC quantitative determination, the linear range of quercetin and kaempferide was in the range of 0.4008 - 3. 2064 μg and 0. 0403 - 3. 2256 μg, respectively. Conclusion: These methods are simple, accurate and reproducible, and can be used to control the quality of Jasmini Sambacis Flos effectively.
出处
《中药材》
CAS
CSCD
北大核心
2013年第5期731-736,共6页
Journal of Chinese Medicinal Materials
基金
广西壮药质量标准研究项目(MZY2010074)
关键词
茉莉花
质量标准
TLC
HPLC
显微鉴别
Jasmini Sambacis Flos
Quality standard
TLC
HPLC
Microscopic identification