摘要
[目的]优化漏芦花总黄酮提取工艺和纯化工艺,为漏芦花抗炎药效部位的开发奠定基础。[方法]以总黄酮为指标,采用UV测定方法,在检测波长413 nm下,测定总黄酮含量,确定纯化漏芦花总黄酮纯化大孔树脂型号;并采用正交试验分别考察乙醇浓度、回流次数、回流时间和乙醇用量对漏芦花总黄酮提取工艺的影响及乙醇体积分数、上样液质量浓度、p H和上样液流速对总黄酮纯化工艺的影响。[结果]确定漏芦花总黄酮提取工艺的最优方案为采用75%乙醇溶液,回流2次,每次回流时间45 min,乙醇用量12倍,影响因素的顺序从大到小依次为乙醇浓度、回流时间、乙醇用量、回流次数。纯化工艺试验中,最优纯化工艺条件为上样液浓度0.05 g/m L,上样液p H为5,上样液流速为3 m L/min,径高比为1∶9,最佳上样量为药材量与树脂用量比2∶1,乙醇体积分数50%,洗脱液用量5 BV;纯化后干膏里漏芦花抗炎有效部位总黄酮含量为42.3%。[结论]AB-8型大孔树脂可有效富集、纯化漏芦花总黄酮,优选的提取和纯化工艺稳定可行。
[Objective] To optimize extraction and purification process of total flavonoids from Rhaponticum uniflorum, to lay a foundation for development of anti-inflammatory parts of Rhaponticum uniflorum. [ Method ] UV was employed to determine the content of total flavonoids with detection wavelength at 413 nm ;using orthogonal design to investigate effects of ethanol concentration, reflux times, reflux time and alcohol dosage on extraction of total flavonoids from Rhaponticttm uniflorum, as well as effects of ethanol volume fraction, mass concentration of sample solution, pH and sample liquid flow rate on purification of total flavonoids. [ Result ] As for extraction process, the optimum scheme was： 12 times extraction solvent dosage, 75% ethanol reflux, extraction for 2 times, each time for 45 minutes. The order of influencing factors was ethanol concentration, reflux time, ethanol dosage and reflux times. As for purification process, optimum technological conditions were as follows ： ratio of diameter-height 1 ： 9, sample solution concentration of 0.05 g/mL, sample solution flow rate of 3 mL/min, pH 5.0, with 5 BV of 75 % ethanol as eluent ： total flavonoids was 42.3 % in anti-inflammatory parts of Rhaponticum uniflorum after purification. [ Conclusion ] Total flavonoids from Rhaponticum＇ s dried flowers could be effectively purified and enriched by AB-8 macroporous resin.
作者
李红
杨晓歌
李霄
LI Hong YANG Xiao-ge LI Xiao(Beijing University of Technology, Beijing 100124)
出处
《安徽农业科学》
CAS
2017年第1期124-127,共4页
Journal of Anhui Agricultural Sciences
关键词
漏芦花
提取
纯化
大孔树脂
总黄酮
Rhaponticum uniflorum
Extraction
Purification
Macroporous resin
Total flavonoids
