摘要
改进了食品中碱性橙检测的高效液相色谱分析方法。采用甲醇提取,Cleanert Alumina N固相萃取柱净化黄鱼和豆制品中的碱性橙,以XDB-C18(5μm,150mm×4.6mm)色谱柱进行分离,检测波长为452nm,以甲醇-0.02mol/L乙酸铵溶液为流动相梯度洗脱。碱性橙的检测线性范围为0.006～100μg/mL,最低检出浓度为0.005mg/L,RSD为3.06%,加标回收率为90.2%～97.5%。
The analytical method of basic orange2 in foodstuffs by reversed phase high performance liquid chromatography（RP-HPLC） was improved.Basic orange2 was extracted by methanol from yellow-fin tuna and bean-product,then purified by Cleanert Alumina N SPE.Separation and quantification was achieved by using a XDB-C18 （5μm, 150mm × 4.6mm）column.And a binary mobile phase of methanol and 0.02mol/L ammoniumv was used with the following gradient elution.A photo-diode array detector was used to detect the compositions with 452nm as the detection wavelength. The linear range of the proposed method was 0.006-100μg/mL, and the detection limit was 0.005mg/L, and RSD was 3.06%, and the recoveries were 90.2%-97.5%.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第1期304-306,共3页
Science and Technology of Food Industry
基金
“十一五”科技支撑项目(2006BAK02A17)
河北省地方标准项目(200585)
关键词
碱性橙
反相高效液相色谱
梯度洗脱
食品
basic orange2
reversed phase high performance liquid chromatography
gradient elution
foodstuffs
