摘要
建立了植物提取物中的碱性橙21、碱性橙22、碱性嫩黄O、罗丹明B、碱性橙2、酸性橙2等6种工业染料的液相色谱-串联质谱分析方法。样品经乙腈提取,固相萃取柱净化,使用Waters Acquity^(TM) UPLC BEH C18柱作为分析柱,以多反应监测(MRM)方式在ESI(±)模式下进行检测,外标法定量。结果表明:6种工业染料含量在5.0~50.0 ng/m L范围内线性良好,相关系数(r^2)均大于0.99;在5,10,25μg/kg 3个添加水平下回收率为65.0%~122.4%,相对标准偏差(RSD)在1.8%~12%(n=6)之间。方适用于植物提取物中多种工业染料的同时测定。
A liquid chromatography- tandem mass spectrometry （LC -MS/MS） method was developed for the determination of six industrial dyes in plant extracts, including alkaline orange 21, alkaline orange 22, Auramine （0）, Rhodamine B, basic orange 2 and Acid Orange 2. Samples were extracted by acetonitrile, purified by solid phase extraction column, and separated on a Waters AcquityTM UPLC BEH C18 column. The MS analysis was performed on electrospray ion source （ESI） in positive and negative ion mode, multiple reaction monitoring （MRM） mode was used for ion signal acquisition, and the external standard method was adopted for quantification. The results showed good linearity in the range of 5.0 - 50.0 ng/mL, with correlation coefficients （r2） higher than 0.99. At the spiked levels of 5, 10 and 25 μg/kg, the average recoveries were in the range of 65.0%- 122.4% with the relative standard deviations from 1.8% to 12% （n =6）. This method is simple and sensitive, and can be used for the simultaneous determination of multiple industrial dyes in plant extracts.
出处
《分析试验室》
CAS
CSCD
北大核心
2016年第12期1402-1407,共6页
Chinese Journal of Analysis Laboratory
基金
国家质检总局科技项目(2014IK120
2015IK216)资助
关键词
固相萃取
超高效液相色谱-串联质谱
工业染料
植物提取物
Solid phase extraction
Ultra performance liquid chromatography trandem mass spectrometry
Industrial dyes
Plant extracts
