摘要
采用固相萃取法提取葡萄酒中氨基甲酸乙酯,然后用气相色谱法测定,色谱条件为:载气流速1.0mL/min;程序升温50℃(1min)→10℃/min→150℃→3℃/min→220℃(5min);进样量2.0μL。分析时间为38min。比较了5种净化柱提取除杂效果,选择CleanertSilica柱作为葡萄酒中氨基甲酸乙酯的萃取柱。以内标法定量,测得相对标准偏差为1.6%、1.2%,回收率在90.4%～101.5%之间。采用本方法测定了8种葡萄酒,氨基甲酸乙酯含量为11.6～30.1μg/L。
Sample was extracted by solid-phase extraction （SPE） and then analyzed by gas chromatography （GC）. The optimal separation conditions were as follows ： flow rate was 1.0 mL/min, column temperature as temperature program： 50 ℃ （1 min）→10℃/min→150℃→3℃/min→220℃（5 min）, sample size was 2.0 μL; The time of determination was 38 min. The efficiencies on extraction of five extraction column were compared and the cleanert silica column was chosen. Internal standard method was used as the quantitative determination. The RSDs were 1.6% , 1.2% and the recovery rates were 90.4% - 101.5% respectively. With this method, 8 wine samples were determined, the contents of ethyl carbamate were 11.6 - 30.1 μg/L.
出处
《上海水产大学学报》
CSCD
北大核心
2008年第5期616-619,共4页
Journal of Shanghai Fisheries University
基金
国家发展和改革委员会计划项目(2007-406)
关键词
固相萃取
气相色谱
葡萄酒
氨基甲酸乙酯
Solid-phase extraction
Gas chromatography
Grape wine
Ethyl carbamate
