摘要
目的:建立了一种用于腐乳中氨基甲酸乙酯含量的同位素稀释-气相色谱-质谱联用检测方法。方法:采用D5-氨基甲酸乙酯同位素稀释技术,C18和硅藻土固相萃取净化样品,然后用GC/MS测定样品中氨基甲酸乙酯。结果:该方法在腐乳中氨基甲酸乙酯的加标回收率在89%～99%之间,相对标准偏差(RSD)小于10%,最低检测限达到2.0μg/kg。结论:方法净化效果好,检测灵敏度、准确度均满足检测要求,可作为腐乳中氨基甲酸乙酯含量检测的确证方法。
Objective：To establish a method for determination of ethyl carbamate in fermented bean curd by isotopic dilution-gas chromatograph-mass spectrometry（GC/MS）.Methods： Ethyl carbamate was determined by gas chromatography-mass spectrometry in selection ion mode after the samples were spiked with D5-ethyl carbamate and purified by C18 and diatomite solid phase extraction column.Results： The recovery rates were 89%~99% and RSD10%,the limit of determination was 2.0 μg/kg.Conclusion： The method is sensitive,accurate and could be used for determination of ethyl carbamate in fermented bean curd.
出处
《中国卫生检验杂志》
CAS
2011年第11期2631-2632,共2页
Chinese Journal of Health Laboratory Technology
