摘要
利用RAPD分子标记技术对中国20份枇杷属植物种质资源及其近缘属植物（石楠和石斑木）进行了遗传亲缘关系及分类研究。从300条10碱基随机引物中筛选出24条引物对20份枇杷种质资源的基因组DNA进行扩增，共产生232条谱带，全部为多态性谱带（多态率为100％），表明枇杷属植物种质之间具有丰富的多态性。各种质间的相似性系数为0．33～0．77，并根据相似性系数，利用UPGMA构建了亲缘关系树状图。在相似性系数为0．485时，20份枇杷属植物及其近缘属可分为3大类：所有的枇杷属植物聚在一起构成第1类，而石楠和石斑木分别构成其余2类，这与传统的分类方法一致；但18份枇杷属种质可分为5个亚类，这与枇杷属植物的传统分类法有所不同。初步推论．开花时间和老叶叶背的茸毛可能不是判断枇杷属植物亲缘关系的必要条件，而且枇杷属植物的进化与其生存的地理环境的变迁有着密切联系。
Random amplified polymorphic DNA markers （RAPDs） have been proposed as valuable tools for determining genetic relationships in plant species. This technique was used to evaluate the genetic relationship of 18 accessions of Eriobotrya and two close relatives Rhaphiolepis indica （L.） Lindl. and Photinieae serrulaia L. The 300 arbitrary primers were screened and 24 primers were selected for amplification of unambiguous and high polymorphic bands. In the RAPD analysis, 232 polymorphic bands were totally generated using 24 arbitrary primers. The 232 bands were all polymorphic and the polymorphism rate was 100%. The results revealed the high genetic diversity in the Eriobotrya genus. The genetic similarity among Eriobotrya plants ranged from 0.33 to 0.77. UPGMA cluster analysis of the similarity matrix obtained from the RAPD database resulted in a dendrogram of genetic relationships that grouped 20 accessions into 3 clusters as the genetic similarity value at 0.485： the 18 accessions of loquat formed a big cluster and the two relatives Rhaphiolepis indica （L.） Lindl and Photinieae serrulaia L. formed individual clusters. This result is consistent with that of the traditional classification in the previous studies, but the classification of sub-clusters of the Eriobotrya plants was somewhat different from that of the traditional classification. We drew a preliminary conclusion that the florescence and the floss of leaf back might not be the necessary conditions to estimate the genetic relationship of the genus Eriobotrya, and that the evolution of the genus Eriobotrya is tie up closely with the changes of its existent geographic environment.
出处
《果树学报》
CAS
CSCD
北大核心
2009年第1期55-59,共5页
Journal of Fruit Science
基金
广东省自然科学基金(06300439)
