摘要
目的:探索采用高效液相色谱法测定大鼠血浆中洛伐他汀浓度的方法。方法:血浆样品采用环已烷-二氯甲烷(3.5:1,V/V)提取,HPLC条件为色谱柱采用XTerra(?)MSC18柱(150×2.1 mm,5μm),流动相为乙腈-水(63L37,V/V),柱温35℃,检测波长为238nm。结果:大鼠血浆中洛伐他汀在0.01～5μg/mL线性范围内线性关系良好,最低检测浓度为0.01～g/mL,方法的提取回收率为81%～95%,日内、日间RSD均小于9.55%;药代动力学结果表明洛伐他汀在雌雄大鼠体内的Tp、Cmax和AUC均有显著性差异(p<0.05)。结论:建立的HPLC方法简便、灵敏度高,重现性好,药动学研究表明洛伐他汀在大鼠体内存在性别差异。
Objective： To investigate the determination of Lovastatin by HPLC in rats after oral single dose. Methods The plasma samples were extracted by cyclohexane-dichloromethane （3.5：1, V/V）. The condition of HPLC： the analytical column was XTerra MS C18 （150× 2.1 mm, 5 μm）. The mobile phase was acetonitrile-water （63： 37, V/V）. The temperature of column was 35 ℃. The compounds were quantified by using an ultraviolet detector operated at 238 nm. Results： The linear correlation of rat plasma lovastatin was good in range of 0.01-5μg/mL. The limit density of detection was 0.01 μg/mL, and the extraction recovery rate was between 81% and 95 %. The phannacokinetics parameter of lovastatin in rats： Tp, Cmax and AUC0-10 were obviously different in different sex rats（p〈0. 05）. Conclusion： This analytical method is simple, high sensitive and high reduplicative enough. The study showed that there were sex differences of lovastatin in rats.
出处
《现代生物医学进展》
CAS
2008年第12期2533-2535,共3页
Progress in Modern Biomedicine
基金
国家高技术研究发展(863)计划项目(2006AA090401)
