摘要
[目的]建立了同时检测红曲类保健食品中美伐他汀、去羟基洛伐他汀的超高效液相色谱-串联质谱法。[方法]样品经甲醇超声提取,采用Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm,1.7μm),流动相为乙腈-0.1%甲酸/水溶液(75∶25),流速0.2 mL/min,以电喷雾离子源正离子多反应模式(MRM)进行检测。[结果]2种目标物在4 min内得到良好分离。美伐他汀和去羟基洛伐他汀分别在6.1~606.6和4.8~483.5 ng/mL范围内与峰面积呈良好的线性关系(r=1),低、中、高3个添加水平的回收率在97%~105%,RSD<3.0%,检出限分别为17、15μg/kg。[结论]该方法简单、快速、灵敏、准确,可用于红曲类保健食品中美伐他汀和去羟基洛伐他汀的同时检测。
[Objective] To establish a method for simultaneous determination of mevastatin and dehydro lovastatin in monascus kinds of health food based on ultra-high performance liquid chromatography-tandem mass spectrometry. [Method] The samples were extracted by methanol. With acetonitrile and 0.1 % formic acid(75∶25) as mobile phase at a flow rate of 0.2 mL/min, the determination was conducted on Waters ACQUITY UPLC BEH C18(2.1 mm×50 mm, 1.7 μm) column by tandem mass spectrometry in positive ESI mode under multiple reaction monitoring(MRM) mode. [Result] The two targets were well separated within 4 min. The calibration curve of mevastatin and dehydro lovastatin was linear in the concentration range of 6.1-606.6 and 4.8-483.5 ng/mL(r=1), respectively. The average recovery were in range of 97%-105% at low, modern and high added levels, while RSD was less than 3.0%. The limit of detection were 17, 15 μg/kg, respectively. [Conclusion] The method is convenient, rapid, sensitive and accurate, which is suitable for simultaneous detection of mevastatin and dehydro lovastatin in monascus kinds of health food.
作者
严俊
颜琳琦
胡磊
陈岑
徐柏杨
周明昊
YAN Jun;YAN Lin-qi;HU Lei(Zhejiang Institute for Food and Drug Control,Hangzhou,Zhejiang 310000)
出处
《安徽农业科学》
CAS
2020年第16期192-194,共3页
Journal of Anhui Agricultural Sciences
关键词
超高效液相色谱-串联质谱法
美伐他汀
去羟基洛伐他汀
红曲类保健食品
同时测定
Ultra-high performance liquid chromatography-tandem mass spectrometry
Mevastatin
Dehydro lovastatin
Monascus health foods
Simultaneous determination
