摘要
目的 探索采用反相高效液相色谱法 (HPLC) ,提高检出血浆洛伐他汀浓度及缩短测定时间。方法 HPLC条件 :色谱柱YWG -C1 8(1 0 μm) ;流动相∶甲醇∶水 (83∶1 7,V/V) ;柱温 2 5℃ ;检测波长 2 37nm。血浆经环己烷和异丙醇萃取浓缩后 ,直接进样测定洛伐他汀的浓度。结果 血浆洛伐他汀浓度在 2 0～ 80 0 μg/L范围内线性关系良好 ,回归方程式 ^y =2 4 1 56 +1 97 2 5x(r=0 9991 ) ;最低检测限为 2 0 μg/L ;平均回收率为 (96 0 1± 7 1 7) %。测定一个样品的时间只需 6min。结论 血浆前处理充分 ,流动相甲醇与水比例适当是提高检测限和缩短测定样品时间的关键 。
Objective To improve the detection concentration and time of plasma lovastatin by studying the RP-HPLC method.Methods The condition of HPLC:The analytical column was packed with YWG-C18(10?μm).The mobile phase was methanol-water(83∶17,V/V).The temperature of column was 25?℃.The compounds were quantified using a ultraviolet detector operated at 237?nm?The lovastatin in plasma sample extracted by cycllohexane-isopropyl alcohol.Results The liner range of plasma lovastatin was 2.0-80?ng/ml.The regression recation was =241 56+197 25x( r =0 999?1).The limit of detection was 2 0?μg/L and the mean recovery was (96 01±7 17)%.The determination of a sample only needed 6?min.Conclusion Controlling the pretreatment of plasma and the ratio of methanol to water was the way to enhance the detection limit and shorten detection time.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2003年第7期814-815,共2页
Chinese Journal of Public Health
