摘要
目的探讨孕酮对缺氧缺血性脑损伤新生鼠皮层和海马糖原合成酶激酶-3β(GSK-3β)水平的影响。方法40只7日龄新生Wistar大鼠随机分成假手术组、缺氧缺血组、孕酮4 mg组、孕酮8 mg组和孕酮16 mg组。假手术组腹腔注射溶剂芝麻油,缺氧缺血组于缺氧前30 min腹腔注射溶剂芝麻油,孕酮组于缺氧前30 min腹腔注射相应剂量的孕酮溶液,建立缺氧缺血脑损伤动物模型。24 h后动物全部处死,采用酶联免疫吸附法(ELISA)检测脑组织中GSK-3β的含量。结果缺氧缺血组新生鼠皮层和海马组织中GSK-3β水平明显高于假手术组(P<0.01),孕酮4 mg、8 mg、16 mg组皮层和海马组织中GSK-3β水平均明显低于缺氧缺血组(P<0.05)。结论缺氧缺血可升高新生鼠皮层和海马GSK-3β水平,孕酮通过降低新生鼠缺氧缺血时皮层和海马组织中GSK-3β水平发挥对缺氧缺血性脑损伤的保护作用。
Objective To study the effect of progesterone on the level of glycogen synthase kinase-3β （GSK-3β） in the cortex and hippocampus of newborn rats with hypoxic-ischemic brain damage. Methods Forty 7-day-old Wistar rats were divided into five groups randomly： sham operation group, hypoxic-ischemic group and progesterone（PROG） 4 mg, 8 mg, 16 mg groups. The rats in sham operation group were injected intraperitoneally with solvent sesame oil, the rats in hypoxic ischemic group were injected intraperitoneally with solvent sesame oil at thirty minutes before hypoxia, and the rats in progesterone group were injected with progesterone solution respectively at thirty minutes before hypoxia to establish the animal models of hypoxic-ischemic brain damage. All rats were killed after 24 hour, then the level of GSK-3β was detected by Enzyme-linked immunosorbent assay （ELISA）. Results The level of GSK-3β in the cortex and hippocampus in hypoxic-ischemic group were significantly higher than that in sham operation group（ P 〈0.01 ） ,and in progesterone 4 mg,8 mg, 16 mg groups was significantly lower than that in hypoxic-ischemic group（ P 〈 0.05 ）. Conclusion The level of GSK-3β in the cortex and hippocampus of newborn rats can be increased by hypoxia-ischemia, and progesterone plays an important role in preventing brain damage from hypoxic-ischemic by reducing the level of GSK-3β.
出处
《新乡医学院学报》
CAS
2008年第6期556-558,共3页
Journal of Xinxiang Medical University
基金
河南省教育厅自然科学研究项目(编号:2008A310013)
新乡医学院科研资助项目(编号:2007YJA42)
