摘要
目的探讨孕酮对缺氧缺血性脑病(HIE)新生鼠脑组织中超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。方法40只7日龄新生Wistar大鼠随机分成5组。假手术组、缺氧缺血组、4 mg孕酮组、8 mg孕酮组和16 m孕酮组。动物先行左侧颈总动脉结扎术,再吸入8%O2和92%N2的混合气体2.5 h建立HIE动物模型。药物组于建立模型前30 min给予不同剂量孕酮溶液,24h后动物全部处死,观察各组新生鼠脑组织中SOD活性和MDA含量的变化。结果缺氧缺血组新生鼠脑组织中SOD活性明显低于假手术组(P<0.01),丙二醛含量明显高于假手术组(P<0.01),药物组SOD活性明显高于缺氧缺血组(P<0.05),丙二醛含量低于缺氧缺血组(P<0.05)。结论孕酮通过升高新生鼠缺氧缺血时脑组织中SOD活性和降低丙二醛含量发挥对缺氧缺血性脑损伤的保护作用。
Objective To investigate the effect of progesterone（PROG） on the SOD activity and the MDA content in brain tis,sue of hypoxic - ischemic encepholopathy （HIE）in newborn rats. Methods Forty 7-day-old neonatal rats were randomly divided into five groups ： sham- operated group, hyloxic -ischemic group and 3 pretreatment groups（4 mg,8 mg , 16 mg progesterone respectively） . The rats were subjected to left eorpanon carotid artery ligation, then were exposed to 8% O2 and 92 % N2 for 2.5 h to establish HIE model. The PROG was injected intraperitoneally into the pretreatment groups rats respectively at 30 min before hypoxia. All rats were killed at the 24 h after operations, then the SOD activity and the MDA contents were mensurated. Results The SOD activity in hypoxic ischemic group was significantly lower than that in the shamoperated group（P 〈 0.01 ）, the MDA contents was significantly higher than that in the sham - operated group（ P 〈 0.01 ）. Compared with the hypoxic - ischemic group, the SOD activity in pre treatment groups was significantly higher（ P 〈 0.05 ）, and the MDA contents in the pre treatment groups was significantly lower （ P 〈 0.05 ） . Conclusion PROK; can increase SOD activity and decrease MDA contents in brain tissue of newborn HIE rats,so it plays an important role in preventing brain against hypoxie- ischemic brain damage by inhibiting the release of free radicals.
出处
《新乡医学院学报》
CAS
2006年第5期450-452,共3页
Journal of Xinxiang Medical University
基金
河南省科技攻关项目(编号:991170318)
河南省教育厅自然科学研究项目(编号:200510472004)
关键词
孕酮
缺氧缺血性脑病
新生鼠
超氧化物歧化酶
progesterone
hypoxic-ischemic entepholopathy
neonatal rats
superoxide dismutase
