摘要
目的观察改构体酸性成纤维细胞生长因子（aFGF）对帕金森病大鼠模型行为学的影响。方法采用SD雄性大鼠72只，随机分为假手术组，PD模型组，PD模型加生理盐水组（PD＋NS组），PD模型加改构体aFGF组（PD＋aFGF组），每组18只。通过脑内立体定向术，将6-OHDA注人后3组大鼠左侧黑质致密部（substantia nigra pars compacta，SNC）和中脑被盖腹侧区（ventral tegmental area，VTA）以建立帕金森病模型，PD＋NS组于术后第1天起每隔2d于右侧侧脑室注射10μl NS，PD＋aFGF组于术后第1天起每隔2d于右侧侧脑室注射10μl改构体aFGF（0．2μg／μl），假手术组只进行假手术处理，分别于术后4d，1，2，3，4周用阿扑吗啡（apomorphine，APO）给大鼠腹腔注射（0．5mg／kg），观察并记录大鼠行为学变化情况。结果假手术组均未出现旋转行为，PD模型组和PD＋NS组大鼠旋转启动时间逐渐缩短，持续时间逐渐延长，旋转速度逐渐加快，至术后2周旋转行为趋于稳定；在术后2周，PD＋aFGF组旋转启动时间[（5．50±1．18）min]，较PD模型组[（3．60±1．17）min，P〈0．05]和PD＋NS组[（3．10±1．02）min，P〈0．05]明显延长；PD＋aFGF组旋转持续时间[（25．90±8．80）min]，较PD模型组[（55．404-10．1,I）min，P〈0．05]和PD＋NS组[（53．904-12．27）min，P〈0．05]明显缩短；PD＋aFGF组旋转平均速度[（6．52±1．34）r／min]，较PD模型组[（12．90±2．21）r／min，P〈0．05]和PD＋NS组[（11．80±3．65）r／min，P〈0．05]明显减慢。术后3周、4周，大鼠旋转行为各项指标统计学分析结果与术后2周相同。结论PD模型大鼠具有明显得行为学改变，改构体aFGF能明显改善大鼠的旋转行为。
Objective To investigate the effects of nonmitogenic aFGF on the behavioral changes of Parkinson＇s disease rat model. Methods Seventy-two male SD rats were divided randomly into 4 groups：Shame group, PD group, PD ＋ NS group and PD ＋ aFGF group, and stereotaxic injection of 6-OHDA into the left substantia nigra pars compacta （SNC） and ventral tegmental area （VTA） of the rats in the latter three groups to develop PD rat model. In the PD ＋ NS group, injected 10 μl NS into the right lateral ventricle of the rats at 2 days interval since 1 day after the injection of 6-OHDA. In the PD ＋ aFGF group, injected 10 μl （0.2 μg/μl） nonmitogenic aFGF into the right lateral ventricle of the rats at 2 days interval since 1 day after the injection of 6-OHDA. In the Shame group,the rats were injected natural saline（NS） instead of 6-OHDA into the left SNC and VTA. Observed and recorded the behavioral changes of the rats at 4 days, 1,2,3,4 weeks after the injection of 6-OHDA. Results No rotary behaviors were observed in the Shame group. In PD group and PD ＋ NS group, the start time of PD rats shortened gradually, the continuous time lengthened, and the rotation rate speeded by degrees. The rotary behavior became invariable until 2 weeks after injection. 2 weeks later, in PD ＋ aFGF group, the start time [ （5.50±1.18 ） min] was longer than the PD group[ （3.60±1.17） min, P 〈0. 05 ] and the PD ＋ NS group[ （3.10±1.02） min, P 〈0.05 ] ; the continuous time [ （25.90±8.80）min ] was shorter than the PD group[ （55.40±10.14）min, P〈 0.05 ] and the PD ＋ NS group [ （ 53.90±12.27 ） min, P 〈 0.05 ] ; the rotation rate [ （ 6.52±1.34 ） r/min ] was slower than the PD group[ （ 12.90±2.21 ） r/min, P 〈 0.05 ] and the PD ＋ NS group [ （ 11.80±3.65 ） r/min, P 〈 0. 05 ]. Compared the rotary behaviors between the three groups, the same statistical results as 2 weeks later can be found at 3 or 4 weeks later. Conclusion PD rats have serious behavioral changes, and
出处
《中国行为医学科学》
CSCD
2008年第11期961-963,共3页
Chinese Journal of Behavioral Medical Science
基金
基金项目：“863”国家基金资助项目（2001AA215131）
