摘要
目的观察微囊化PC12细胞移植后帕金森病大鼠旋转行为的变化。方法采用免疫隔离微囊技术将能分泌多巴胺的PC12细胞包裹后,移植到帕金森病大鼠脑内,观察阿扑吗啡诱发病鼠旋转行为的变化,酪氨酸羟化酶(TH)抗体免疫组化染色观察移植区阳性细胞的表达。结果帕金森病大鼠旋转行为有明显改善,由移植前(11.88±2.04)圈/min减少为移植后(3.46±1.01)圈/min。移植后3个月,大鼠脑内微囊完整,仍有TH阳性PC12细胞存活。结论微囊化PC12细胞移植可明显改善帕金森病大鼠的旋转行为。
Objective To observe the change of the rotational behavior in Parkinson g disease （PD） rats after implantation of microencapsulated PC12 cells. Methods A new method of immunoisolation （microcapsulation） was utilized for encapsulation of dopamine-secreting PC12 cells, and the cells were implanted into the brains of PD rats. Then the change of apomorphine-induced rotational behavior of the PD rats was analyzed. Tyrosine hydroxylase （TH） antibody immunohistochemistry stains of the cells in the implanted area were also proceeded. Results The rotational behavior of the PD rats reduced significantly. The turns of preimplantation and postimplantation 3 months were 11. 88± 2.04 rpm and 3.46 ±1.01 rpm, respectively. The microcapsules in the brains of PD rats were intact and TH immunopositive PC12 cells could exist for 3 months postimplantation. Conclusion The rotational behavior of the PD rats can be ameliorated after implantation of microcapsulated PC12 cells.
出处
《中华神经外科疾病研究杂志》
CAS
2006年第5期410-413,共4页
Chinese Journal of Neurosurgical Disease Research
基金
Supported by the Natural Science Foundation ofChongqing City (CSTC-2004B135038)
关键词
微囊技术
PC12细胞
移植
帕金森病
旋转行为
Microencapsulation
PC12 cell
Implantation
Parkinsong disease
Rotational behavior
