摘要
目的 观察毁损黑质多巴胺能神经元大鼠行为学及其形态学变化特点 ,探讨两者之间的相关性。方法 利用 6 -羟基多巴胺 (6 - OHDA)单侧一点注射大鼠黑质致密区 (SNc) ,特异毁损多巴胺 (DA )能神经元 ,采用开野实验观察术后 1d、3d、5 d、7d、14 d、2 1d行为学变化 ;利用 Nissl染色、HE染色、免疫组织化学方法和电镜的方法 ,观察各时间点黑质形态学变化。结果 毁损侧 DA能神经元逐渐减少 ,超微结构损伤逐渐加重 ;开野实验中旋转、探究、后肢站立和穿梭行为在术后 1d即有显著改变 (与对照组比较 P<0 .0 5 ) ,其中 ,旋转行为与毁损程度呈正相关 (r=0 .4 71,P <0 .0 1) ,探究、后肢站立和穿梭行为与毁损程度呈负相关 (r分别为 - 0 .719、- 0 .5 89、- 0 .5 94 ,P <0 .0 1) ,修饰行为与毁损程度无相关性 (r=- 0 .2 2 7,P>0 .0 5 )。结论 黑质 DA能神经元丢失是毁损大鼠行为改变的病理学基础 ,开野实验可作为丢失程度的敏感行为学观察指标。
Objective To observe the characteristics of praxiology and morphology of 6-hydroxydopamine(6-OHDA) lesioned rats and the relation between them. Methods Rats received a unilateral injection of 6-OHDA into the substantia nigra pars compacta (SNc). Behavioral changes and dopaminergic degeneration were observed with the open-field test,HE and Nissl staining,eletromicroscopic and immunocytochemistry methods at 1d,3d,5d,7d,14d,21d after lesion. Results The number of dopaminergic neurons decresesed gradually in the lessioned site and its electromicroscopic structure was severe by degrees. All parameters of open-field test except grooming had a notable alternation 1d after lesion (P< 0.05). The correlational analyses yielded a positive relationship between turning and lesion size (r=0.471,P< 0.01),while scanning、rearing and locomotor activity were negatively correlated with lesion size (r=-0.719,r=-0.589,r=-0.594,P< 0.01). Conclusion Dopaminergic neurons deletion makes the foundation of behavioral changes in 6-OHDA lesioned rats. The open-field test is useful to predict lesion size.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2005年第1期37-40,i002,共5页
Journal of Apoplexy and Nervous Diseases
基金
安徽省科技厅重点项目 (项目编号 :10 41184)
