摘要
目的阐明棉酚造成精子质量下降的生化机制,为其应用和降低毒副作用提供新思路。方法采用Wistar大鼠口服给予棉酚(50mg·kg-1/2d),2wk后取附睾尾精子,采用CASA进行检测,同时对血清中的促黄体生成素(LH),促卵泡生成素(FSH)、睾酮(T)进行检测,对睾丸组织形态学以及组织内的一氧化氮(NO)含量进行检测。结果棉酚可造成精子的数量减少,活力降低。睾丸组织内部结构紊乱,睾丸组织中的NO水平上升,血清中的T下降,而对FSH、LH无影响。化合物NA1108可拮抗棉酚造成的精子质量下降,降低睾丸内NO含量,升高血清中T含量。结论棉酚造成精子质量降低的机制之一是由于其造成睾丸组织内NO的过量产生,从而损伤睾丸组织内的多种细胞所致,能降低睾丸内NO含量的药物可提高精子质量。
Aim To elucidate the biochemical mechanism of gossypol in inducing the decline of sperm quality. Methods Gossypol was administered orally at thedose of 50 mg · kg^-1/2 d for two weeks. Then, the sperm was eolleeted from the left caudal epididymis and was analysed by CASA. The morphological ehangeand the concentration of nitric oxide （NO） in testes as well as the level of hormone [ follicle-stimulating hormone （FSH） ], luteotrophic hormone （ LH ）, testosterone （T） in serum were assayed. Results Gossypol could induce the decrease of sperm number and sperm quality. The concentration of NO in testes increased significantly. Among the three kind of hormone, only the concentration of T showed decrease after the oral administration of gossypol. NAll08 could antagonize the de-cline of sperm quality damaged by gossypol and decrease the content of NO in testes. Conclusions The concentration of NO in testes beyond normal value was one of the toxic mechanism of gossypol that contributed to the inhibition of spermatogenesis. Some drugs with the ability to reduce NO content in testes could also increase sperm quality.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2008年第11期1518-1521,共4页
Chinese Pharmacological Bulletin
关键词
棉酚
精子
一氧化氮
睾酮
gossypol
sperm
nitric oxide
testosterone
