摘要
目的 :探讨锌对外源性一氧化氮 (NO)供体硝普钠 (SNP)损伤精子的保护性作用。 方法 :利用相差显微镜及单细胞凝胶电泳分析锌对SNP影响精子运动能力、精子头尾连接及精子DNA链断裂的保护性。 结果 :SNP对精子运动能力有明显的抑制作用 ,精子彗星细胞百分率显著增加 ,精子头尾连接稳定百分率降低 ,而锌离子能提高精子的运动功能 ,防止精子DNA链的断裂和精子头尾连接的解离。 结论 :锌对NO损伤精子有一定的保护作用 ,其机制可能与精子核染色质部位的巯基有关。
Objective: To study the function of zinc in preventing human sperm from being damaged by sodium nitroprusside(SNP),an external NO donor. Methods: Analyses were made of the function of zinc in protecting sperm from being influenced by SNP in such aspects as sperm motility,head-tail connection and the breakage of sperm DNA chain by using phase-contrast microscope and single cell gel electrophoresis(SCGE). Results: Sperm motility was obviously inhibited by SNP. The percentage of comet cells increased significantly but the stability of sperm head-tail connection decreased. Zinc could promote sperm motility,protect the DNA chain and prevent the sperm head-tail connection from breaking. Conclusion: Zinc can protect sperm from being damaged by NO. Its mechanism may be related to the mercaptol group of sperm chromatin.
出处
《中华男科学杂志》
CAS
CSCD
2004年第7期530-532,537,共4页
National Journal of Andrology
关键词
锌
精子
一氧化氮
硝普钠
DNA
zinc
sperm
nitric oxide
sodium nitroprusside
DNA
