摘要
目的:采用不同的留置时间建立大鼠C6胶质瘤模型,比较不同的留置时间对于模型成功率的影响,提高肿瘤种植成功率。方法:36只SD大鼠按留置时间3min、5min、10min分为3组,C6细胞体积10μl细胞悬液、注射时间5min。第一次未种植成功者再次种植。术后用骨蜡封骨窗并清创缝合。造模术后2周和3周分别行MRI平扫及增强证实模型肿瘤生长。统计学方法采用Fisher精确检验。结果:36只大鼠,16只第一次种植成功,20只种植失败,改变条件再次种植,2只死于麻醉意外,18只成功。留置时间3min与5min,其0.01<P<0.05,留置时间对于肿瘤种植成功二者差异具有统计学意义,留置5min肿瘤成功率高于3min;留置时间5min与10min,其P<0.01,留置5min与10min两种不同的留置时间对于肿瘤种植成功二者差异具有显著的统计学意义,留置10min,肿瘤成功率明显高于5min。结论:微量注射器的留置时间与肿瘤种植成功率有关,随着留置时间的延长,肿瘤种植成功率也会提高。
Objective：To compare different detaining time of the rat model by C6 gliomas implanted and to raise the achievement ratio of the model.Methods：36 rats were divided into 3 groups according to detaining time.Group 1：The detaining time was 3min;group 2 was 5min;and group 3 was 10min.The volume of C6 cell was 10μl and injection time was 5min for each group.The tresis vulnus was sealed with bone wax.Plain scan and enhanced MRI were performed from 2 weeks to 3 weeks after operation.Statistical analysis was performed by using Fisher analysis for comparison of three data,and P values were calculated for each comparison.P≤0.05 was considered to indicate a statistically significant difference.Results：Of the thirty-six rats glioma models,sixteen rats were modeled successfully in the first time;twenty rats were modeled again by changing condition;two rats were dead for anesthetic accident;eigteen rats were planted successfully.There was a significant difference in the achivement ratio among the three groups：（0.01P〈0.05）,which showed the achievement ratio of group 3 （detaining time was 10 minutes） was highest. Detaining time of microinjector was very important in the rat model by C6 gliomas implanted and longer detaining time can raise the achievement ratio of the model.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第10期1260-1263,共4页
Journal of Chongqing Medical University
关键词
C6细胞
SD大鼠
模型
胶质瘤
C6 cell
SDRat
Model
Glioma C6 cell
SDRat
Model
Glioma
