摘要
目的 :评价磁共振成像及其波谱技术在大鼠胶质瘤模型研究中的应用价值。方法 :大鼠C6脑胶质瘤细胞体外培养 ,应用立体定向架将肿瘤细胞注入大鼠脑尾状核区域 ,接种后 12～ 18d分别行磁共振成像及其波谱检查 ,观察大鼠脑C6胶质瘤的磁共振成像及其波谱表现 ,并与病理学变化进行对照分析。结果 :2 4只接种大鼠脑内有 16只胶质瘤生长良好 ,磁共振成像表现为长T1、长T2 信号 ,增强后肿瘤强化明显 ,呈均匀强化或环状强化 ;磁共振波谱表现为NAA峰明显下降甚至消失 ,Cho升高为第一高峰 ,NAA/Cr比值明显下降、Cho/Cr比值明显上升 ,部分肿瘤出现乳酸峰 (Lac)或脂质峰(Lip)。病理学显示肿瘤呈浸润性生长 ,边界不清。随着肿瘤增大 ,肿瘤内部坏死明显 ,乳酸峰 (Lac)或脂质峰 (Lip)出现率增加。结论 :大鼠脑C6胶质瘤模型是一种生长稳定、较为理想的脑胶质瘤模型 ;磁共振成像 (MRI)可以准确提供胶质瘤模型的病理形态学信息 ,磁共振波谱 (MRS)则可以提供胶质瘤的代谢及生化信息。
Objective:To observe manifestations of magnetic resonance imaging (MRI) and 1H magnetic resonance spectroscopy ( 1H MRS) in rat brain C 6 glioma,correlative studies were performed between the manifestations of MRI and 1H MRS with histopathology.Methods:Rat C 6 glioma cells were cultured in vitro and prepared for intracerebral injection.cells were injected into the caudate nucleus of rat brain under guiding of stereotaxic apparatus to establish a rat brain glioma model.MRI and 1H MRS were conducted 12～18 days after implantation.The manifestations of MRI and 1H MRS were correlated with pathologic appearances.Results:16 brain glioma were developed in 24 rats after C 6 cell implantation.All rat brain C 6 gliomas presented as long T 1 and long T 2 signals on MRI and demonstrated obvious homogeneous or annular enhancement after the administration of gadolinium. 1H MRS showed that the NAA peak decreased or disappeared and NAA/Cr ratio decreased and Cho/Cr ratio increased obviously in C 6 glioma.Some of gliomas presented with Lac and/or Lip peak.Histopathologic analysis revealed that all the tumor growed invasively.As the volume of tumor increased,massive necrosis developed in the central area of tumor and the occurrence rates of Lac and/or Lip peak had increased in these lesions.Conclusion:Rat brain C 6 glioma is a very stable and ideal animal model for experimental glioma studies.MRI can provide its pathomorphologic information precisely and MRS can provide its metabolic and biochemical changes in vivo.
出处
《放射学实践》
2004年第12期913-916,共4页
Radiologic Practice
关键词
脑肿瘤
动物
实验
磁共振成像
磁共振波谱学
Brain neoplasms
Animals,laboratory
Magnetic resonance imaging
Magnetic resonance spectroscopy
