摘要
目的本研究通过对大鼠C6/SD脑胶质瘤模型分不同剂量单次全脑放疗后,观察大鼠生存状态及生存期改变,为胶质瘤模型施行综合治疗提供动物实验基础。方法雌性SD大鼠,随机分为假照射荷瘤鼠组和5、10、15、20 Gy4个荷瘤鼠照射组,采用立体定向法在大鼠右侧尾状核接种C6细胞建立大鼠C6胶质瘤模型,于建模后第17天行相应剂量单次全脑放疗,观察各组大鼠生存状态及生存期改变的实验研究。结果①SD大鼠右侧尾状核立体定向方法建立的C6脑胶质瘤模型确切可靠,未见远处及颅外转移。②荷瘤大鼠受照射后第1周内出现体重增加,全身营养状况改善;2周后大鼠自发活动明显减少,饮食量减少,体重下降,照射剂量越低生存期越短,恶液质出现越早;而且3周时15、20 Gy照射组大鼠脑照射区皮肤有脱毛反应,为不全脱毛,无明显充血。③各组大鼠的生存时间:假照射组、5、10、15、20 Gy组的MeST分别为24、30.5、37.5、49、63 d。四个照射组的生存期与假照射组比较差异均具有统计学意义(P<0.05)。结论①脑内接种1×106个C6细胞建立的模型用于治疗实验研究时,治疗处理时间以细胞接种后第2～3周为宜。②荷瘤鼠全脑放疗后,首先出现较短的兴奋亢进过程,然后转入长时间的抑制过程,随着放射剂量的增加,荷瘤鼠生存期延长,说明放射线具有治疗脑胶质瘤作用。
Objective To investigate the C6 glioma rats model and observe the survival states and overall survival days after single whole-brain irradiation with different doses in rats,and establish a basis for glioma combined-therapy from animal experiment.Methods Female SD rats were randomly divided into sham irradiation group and brain-baring C6 glioma groups with radiation by 5,10,15 and 20 Gy.The models of C6 glioma were established by stereotaxical injection of C6 glioma cells into the right caudate nuclei in female SD rats.The rats with brain-baring C6 glioma were radiotherapied with whole brain at 17th day,and then observed the survival status and recorded the survival time of all groups.Results ① Using stereotactic methods to establish the C6 glioma model was exact and reliable without distant and extracranial metastasis.② The weight and nutritional status of tumor-bearing rats gained general improvement within 1 week after irradiation.The spontaneous activity,eating and drinking of rats was significantly reduced,and the weight gradually decreased after 2 weeks.The lower the radiation dose,the shorter survival time and sooner cachexia occurred.At 3 weeks,brain irradiation area were incompletely shed reaction in 15 Gy and 20 Gy irradiated groups,which hadn＇t obvious hyperemia.③ The MeST of sham irradiation group,5 Gy group,10 Gy group,15 Gy group and 20 Gy group was 24 d,30.5 d,37.5 d,49 d and 63 d,respective.The survival time of the four exposure groups were significantly different from the sham exposure group（P 0.05）.Conclusion The model implanted with 1×106 C6 gliomaous cells sterotactically into intracerebral was used for the treatment of experimental studies,and the appropriate time to the treatment was 2 to 3 weeks after cells inoculation.After whole-brain radiotherapy,rats expressed an exited graduation for a few time firstly,then turn to a long time with depressed status.With the role of radiation to treat cancer,with the development of irradiation doses can extend survival time of rats,which in
出处
《解剖学研究》
CAS
2011年第1期4-8,F0003,共6页
Anatomy Research
