摘要
提出了茶叶中5种有机氮农药（速灭威、残杀威、呋喃丹、甲萘威、抗蚜威）残留的高效液相色谱法。样品用丙酮-正己烷（1＋2）混合溶剂提取，经石墨碳固相小柱净化，以VP-ODS C18柱分析，乙腈-60mmol·L^-1磷酸（40＋60）溶液为流动相，流速为1．0mL·min^-1，检测波长210nm，外标法定量。在0．10～2．0mg·kg^-1之间的添加水平，5种有机氮农药的平均回收率在80．1％～94．8％之间，相对标准偏差在2．21％～7．13％之间，检出限在0．0125-0．05mg·kg^-1之间。
A methd based on solid-phase extraction （SPE） combined with HPLC for the determination of residual organonitrogen pesticides, namely, metolcarb, propoxur, carbofuran, carbaryl and pirimicarb, in tea was proposed. Pasticides in sample were extracted with solvent mixture of acetone-hexane （vol. ratio 1 ： 2）, purified on a SPE column and eluted with acetonitrile-toluene mixture （vol. ratio 4 ： 1）. HPLC analysis was carried out on a VP-ODS C18 column using a mobile phase of acetonitrile and 60 mmol · L^-1 phosphoric acid solution mixture （vol. ratio 40 ： 60）, and detected at 210 nm. At concentration levels between 0. 10 and 2. 0 mg· L^-1 , recoveries of these pesticides were in range of 80. 1%-94.8% and RSD＇s in the range of 2. 21%-7. 13%. DL＇s were in the range of 0. 012 5-0. 05 mg· L^-1.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2008年第10期947-949,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
基金
国家科技支撑计划(2006BAD06B01)
