摘要
采用高效液相色谱法测定茶叶中多菌灵和甲萘威农药残留。通过乙腈提取凝胶渗透色谱净化(GPC)和在线浓缩方式预处理;样品检测条件:色谱柱为WatersC18柱(3.0×250mm,5μm),采用乙腈和水为流动相进行梯度洗脱,柱温30℃,流速0.5mL/min,PDA检测器,检测波长为285.2nm和220.3nm,进样量5μL。回收率为84.5%～103.2%;RSD为3.2%～4.5%;多菌灵的农药检测限为0.1μg/mL、甲萘威的农药检测限为0.03μg/mL。
To determine the residues of carbofuran and carbaryl in tea by GPC-HPLC.The sample was extracted by acetonit ester,purified by gel permeation chromatography(GPC),separated with Waters C18 column(3.0 mm×250 mm×5 μm),water and acetonit rile as mobile phase with gradient elution at column temperature of 30,and a flow rate of 0.5 mL/min,detected with PDA detector,and the wavelength was 285.2 nm and 220.3 nm,and the inpouring was 5μL.The recoveries were 84.5 %～103.2 %,corresponding RSDs were 3.2 %～4.5 % and the detection limit of carbofuran was 0.1μg/mL、the detection limit of carbaryl was 0.03μg/mL
出处
《质量技术监督研究》
2009年第2期19-21,共3页
Quality and Technical Supervision Research
