摘要
采用快速溶剂提取前处理技术与表面增强拉曼光谱(surface-enhanced Raman spectroscopy,SERS)检测技术建立豆芽中6-苄基腺嘌呤(6-benzylaminopurine,6-BA)残留物的快速定量检测方法。以自发豆芽为空白对照,通过对梯度浓度的6-BA插标豆芽提取液进行SERS检测发现,在1 002cm-1处具有明显的6-BA特征拉曼峰,且归一化处理后的特征峰峰强与6-BA浓度呈良好的线性关系,高浓度与低浓度的线性范围分别为0.5～14μg.mL-1与0.1～2μg.mL-1,最低定量检出限为0.02mg.kg-1,回收率为82.3%～95.1%,相对标准偏差小于5%。该方法重现性较好、前处理简单、全程检测耗时短、设备操作简便,为市场大规模的快速检测提供了现场、高效、灵敏的解决方案。
New method for rapid and quantitative analysis of 6-benzylaminopurine（6-BA） residues in sprout beans was studied by using FAST-SPE and portable surface-enhanced Raman spectroscopy（SERS）.With homemade sprout bean samples as blank control,6-BA solutions extracted from inserted-treatment samples showed typical characteristic Raman peaks at 1 002 cm-1 tested by SERS,and normalized 1 002 cm-1 intensities had a good linear relationship with gradient concentrations of 6-BA insert-standard solutions.The high concentration linear range was 0.5~14 μg·mL-1,and the low one was 0.1~2 μg·mL-1.The quantitative detection limit was down to 0.02 mg·kg-1 that was below the maximum allowable residues（MAL） of 0.2 mg·kg-1 in related National Standard.The recoveries were 82.3%~95.1% and the RSD was below 5%.This method with portable equipment and simple pre-treatment showed good reproducibility,short test time-consuming and easy operating,and thus it would provide efficient and sensitive solutions for large-scale on-site and rapid detections.
出处
《光谱学与光谱分析》
SCIE
EI
CAS
CSCD
北大核心
2012年第5期1266-1269,共4页
Spectroscopy and Spectral Analysis
基金
国家自然科学基金项目(21107005)
教育部博士点基金项目(3C015001201201)
北京工业大学博士科研启动基金项目(01500054R1752)资助
