摘要
建立了液相色谱法同时测定茶叶中啶虫脒和多菌灵2种农药的分析方法。茶叶粉碎后经乙腈提取后,Carbon/NH2小柱净化,紫外检测器测定,外标法定量。对比NH2和Carbon/NH2固相萃取柱净化效果,对样品前处理和色谱分离条件进行优化,综合考虑,确定茶叶中啶虫脒和多菌灵2种农药的检测方法。结果表明院2种混合农药含量在0.01~0.50 mg/L间呈良好的线性关系,相关系数r2=0.999以上。添加0.05~1.00 mg/L浓度,平均回收率在77.5%~83.4%之间,相对标准偏差（RSD）为5.5%~7.2%之间。方法最低检出限（S/N=3）啶虫脒0.01 mg/kg、多菌灵0.03 mg/kg。该方法的灵敏度、准确度和精密度均符合农药残留测定的技术要求。
A method based on liquid chromatography method was established for the simultaneous determination of two kinds of pesticides tea acetamiprid and carbendazim.Tea after crushing was extracted with acetonitrile, and purified with CarbordNI-I2 column and UV detector,using external standard method.Comparison of NH, and carbon/NH2 solid-phase extraction column purification effect,the sample pretreatment and the chromatographic conditions were optimized, considering the determined in tea of acetamiprid and bacteria spirit 2 kinds of pesticide detection method.The results showed that the contents of 2 kinds of mixed pesticides had a good linear relationship with 0.01-0.50 mg/L,and the correlation coefficient was above 0.999. When the concentration was 0.05-1.00 mg/L, the average recovery rate was in 77.5%-83.4%, the relative standard deviation （RSD）was 5.5%-7.2%. The detection limit （S/N=3）of acetamiprid 0.01 mg/kg and carbendazim 0.03 mg/kg.The sensitivity, accuracy and precision of the method were in accordance with the technical requirements of pesticide residue determination.
作者
冯敏铃
李盛安
王丽娜
张益文
贾晓菲
刘铭扬
张忆萍
FENG Min-ling LI Sheng-an WANG Li-na ZHANG Yi-wen JIA Xiao-fei LIU Ming-yang ZHANG Yi-ping(Zhongshan Agricultural Products Quality Supervision ＆ Test Institute in Guangdong Province,Zhongshan Guangdong 52840)
出处
《现代农业科技》
2016年第19期122-123,127,共3页
Modern Agricultural Science and Technology
基金
中山市科技计划项目(2015B2350)
关键词
茶叶
啶虫脒
多菌灵
液相色谱法
tea
acetamiprid
carbendazim
liquid chromatography
