摘要
报道一种全血无酚法制备染色体ＤＮＡ技术。其特点是：不预先分离白细胞或细胞核，用全血直接提取ＤＮＡ；用饱和ＮａＣｌ代替经典法中的酚／氯仿有机溶剂。所制备的染色体ＤＮＡ２６０／Ａ２８０比值为１．８０～１．８５。每毫升外周血可获得２０μｇ～２８μｇＤＮＡ。该法是一种简便、安全。
We describe in this paper a simple and efficient procedure for preparing peripheral blood DNA without preisolation of white blood cells.The method also avoids the hazard of organic solvent extraction.Routinely it yields 20 to 28μg of chromosomal DNA from per ml whole blood.The A260/A280 ratio for DNA sample is 1.80 to 1.85.The undigested DNAs migrate above the 21kb pair,while the endonucleasedigested samples appear characteristically in the smear form.These data illustrate the consistency and quality of the chromosomal DNA isolated using this procedure.The DNA prepared by this method was used for analysis of βthalassemia mutation and prenatal diagnosis in our laboratory.
出处
《华西医科大学学报》
CSCD
1997年第4期448-449,453,共3页
Journal of West China University of Medical Sciences
基金
国家计划生育委员会科研基金
关键词
全血
无酚法
染色体
DNA
Whole blood Nonphenol method Chromosomal DNA
