摘要
目的探讨D7S2 1位点在河北汉族人群分布的多态性 ,为DNA指纹数据库的构建及其法医学应用提供基础资料。方法应用MVR PCR方法和聚丙烯酰胺梯度凝胶电泳银染法对 12 4名河北汉族人群无关个体D7S2 1位点进行了快速检测 ,并进行数字编码。结果每一个体平均得到 3 6个数字编码 ,未发现任何两个无关个体所有编码相同 ,两无关个体 3 6个编码相同的机率为 3 .4 8× 10 -18。三种重复单位a 型、t 型和o 型出现的机率分别为 4 8.5 %、4 9.5 %和 2 .1%。该位点杂合度为 0 .9876,非父排除率为 0 .974 6,多态性信息含量为 0 .9872。结论D7S2 1位点在河北汉族人群中具有高度的多态性 ,聚丙烯酰胺梯度凝胶电泳银染法简便、快速 。
ObjectiveTo study the polymorphism at D7S21 locus in Hebei Han population,provide basic information for construction of DNA fingerprint database and apply these to the practice of forensic medicine.Methods124 unrelated individuals were detected rapidly by MVR PCR and polyacrylamide gradient gel electrophoresis followed by silver staining,and digital codes were obtained.ResultsEach individual obtained about 36 digital codes.No two unrelated individuals shared the same codes.The probability of identity with 36 digital codes was 3.48 ×10 -18 .The percentage of three repeat units,a type,t type and o type was 48.5 % , 49.4 % and 2.1 % respectively.The heterozygosity (H)?excluding probability of paternity (EPP) and polymorphism information content (PIC) were 0.9876 , 0.9746 and 0.9872 respectively.ConclusionD7S21 locus had highly polymorphism in Hebei Han population.The method polyacrylamide gradient gel electrophoresis followed by silver staining was simple,rapid and had important practice values.
出处
《河北医科大学学报》
CAS
2001年第6期321-324,I001,共5页
Journal of Hebei Medical University
