摘要
为研究D7S2 1基因座在河北汉族人群分布的多态性 ,应用MVR -PCR (MinisatelliteVariantRepeat -PolymeraseChainReaction)方法和聚丙烯酰胺梯度凝胶电泳银染法对 12 4名河北汉人无关个体D7S2 1基因座进行了快速检测 ,并进行数字编码。每一个体平均得到 36个数字编码 ,未发现任何两个无关个体所有编码相同 ,两无关个体 36个编码相同的概率为 3.48× 10 -18。三种重复单位a型、t型和 0型出现的概率分别为 :48.5 %、49.4%和 2 .1%。该基因座杂合度为 0 .9876 ,非父排除率为 0 .9746 ,多态性信息含量为 0 .9872。研究表明 ,D7S2 1基因座在河北汉族人群中具有高度的多态性 ,聚丙烯酰胺梯度凝胶电泳银染法简便、快速 ,具有一定的实用价值。
To study the polymorphism at D7S21 locus in Hebei Han population,124 unrelated individuals were detected rapidly by Minisatellite Variant Repeat Polymerase Chain Reaction (MVR PCR) and polyacrylamide gradient gel electrophoresis followed by silver staining,and digital codes were obtained.About 36 digital codes were obtained from each individual.No two unrelated individuals shared the same codes.The probability of identity in 36 digital codes was 3.48×10 -18 .The percentage of three repeat units,a type,t type and 0 type was 48.5%,49.4% and 2.1% respectively.The heterozygosity (H),excluding probability of paternity(EPP)and polymorphism information content(PIC)were 0.9876,0.9746and 0.9872 respectively.The results suggested that D7S21 locus has highly polymorphism in Hebei Han population.The method polyacrylamide gradient gel electrophoresis followed by silver staining was simple,rapid and practical.
出处
《遗传》
CAS
CSCD
北大核心
2002年第3期242-246,共5页
Hereditas（Beijing)
基金
河北省科技攻关计划项目 (批准号 992 76 12 7)
