摘要
目的:建立2型糖尿病患者唾液蛋白质双向电泳图谱,观察2型糖尿病患者与正常人唾液蛋白质差异表达情况。方法:以优化的双向电泳技术分离唾液蛋白质,银染后用专业软件进行分析,建立2型糖尿病患者与正常人唾液蛋白质双向电泳图谱,并采用二维电泳图谱专用软件对所得结果进行图谱分析。采用基质辅助激光解吸电离-飞行时间质谱法对两组间比较所得目标蛋白质点进行质谱鉴定。结果:分别建立了正常人和2型糖尿病患者唾液蛋白质双向电泳图谱。结论:对比正常人和2型糖尿病患者唾液蛋白质图谱,鉴定出7种差异蛋白,为进一步研究其发病机制及治疗手段提供实验基础。
Objective：The purpose of this study was to establish two dimensional gel electrophoresis（2-DE） profiles of saliva of Type 2 diabetes patients,and to identify the differential proteomic expressions between normal persons and Type 2diabetes patients. Methods：Samples of saliva protein were separated by two dimensional electrophoresis with optimization.Differential proteomic expressions between the Type 2diabetes patients and the normal control persons were identified by two dimensional polyacrylamide gel electrophoresis,silver staining,image master 2-DE software analysis,peptide mass fingerprinting based on matrix assisted laser desorption/ionization time of flight mass spectrometry（ MALDI-TOF-MS ）, Bioworks and NCBI software database searching. Results：The two dimensional polyacrylamide gel electrophoresis profiles of saliva proteins were successfully established by 2-DE. twenty of the significant differential proteins were selected and identified by MALDI-TOF-MS. seven of them were finally identified. Conclusions：The 2-DE profiles of saliva proteins were established and the differential proteomic expressions were identified by proteome technique in our study. This can be an experimental basis for further research of the pathogenesis and treatment of Type 2 diabetes.
出处
《重庆医科大学学报》
CAS
CSCD
2008年第8期959-962,共4页
Journal of Chongqing Medical University
