摘要
通过登革病毒包膜蛋白基因序列测定与进化分析,确定2004 2010年间福建省登革病毒的主要来源地。方法提取登革病毒分离株的RNA,扩增病毒外膜蛋白基因,扩增产物经TA克隆后提取质粒并测序,应用相关专业软件做序列分析。结果在测定的12株登革病毒外膜蛋白基因核酸序列中,1、2型病毒的序列长度均为1 485bp,3型为1 479bp。BLAST比对与序列进化树均表明,2004-2010年期间,福建省所分离的登革病毒分离株与东南亚一带流行的毒株一致性最高,福建省与东南亚流行的毒株间具有高度的同源性。结论2004-2010年福建省流行的登革病毒应当是由东南亚一带输入,应加强对该地区入境人员的监测工作。
Since several dengue viruses（DENV） have been isolated in Fujian Province during the past decade,sequencing and evolution analyses of viral envelope genes are helpful in determining their possible transmission origins.In this study,viral RNA was extracted from 12 DENV strains from Fujian between 2004-2010.Viral envelope genes were amplified,cloned into TA vectors and sequenced,and the sequence data were subsequently analyzed by bioinformatics software.Full-length E genes of DENV-1 or DENV-2 of 1 485 bp,and DENV-3 of 1 479 bp were obtained.It was indicated,from BLAST analysis and phylogenetic trees,that DENV strains in Fujian Province during 2004-2010 shared the highest similarity with Southeast Asian strains,suggesting that DENV circulating in Fujian Province between 2004-2010 were probably imported from Southeast Asia.Hence,extensive monitoring on passengers from this region at the entry-ports should be strengthened.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2012年第10期973-977,共5页
Chinese Journal of Zoonoses
基金
Supported by the Priority Programs in Science and Technology,Fujian Province(No.2010Y0010)
the Priority Programin 863Project in China(No.2011AA02A114)~~
