摘要
为了便于转基因植株的分子检测,利用重叠延伸PCR(gene splicing by overlap extension PCR,SOE PCR)方法克隆来自于水母的绿色荧光蛋白基因(GFP)和来自于拟南芥的目的基因DREB1C转录因子,并对其进行改造获得融合基因。通过酶切和连接,将融合基因构建到表达载体pCAMBIA1301上,获得了具有DERB1C和GFP基因的重组表达载体pDR1-GFP,酶切和测序结果表明,该融合基因与设计相符。
In order to identify transgenic plants at molecular level conveniently, the transcription factors gene （DREB 1 C） from A rabidopsis thaliana and green fluorescent protein gene （GFP） from jellyfish were cloned, modified and fused by gene splicing by overlap extension PCR （SOE PCR）, then fusion gene was cloned into the plant expression vector pCAMBIA 1301 to get the recombinant expression vector pDR 1-GFP by restriction enzyme digestion and ligation, and it was confirmed by DNA sequence analysis.
出处
《分子植物育种》
CAS
CSCD
2008年第3期603-607,共5页
Molecular Plant Breeding
