摘要
从番茄基因组中克隆LeNCED1基因5′-侧翼1456 bp调控序列,经生物信息学分析表明,LeNCED1基因上游调控序列中存在响应昼夜节律的顺式作用元件Circadian、光响应元件Box I、逆境胁迫响应元件(TC-rich repeats)、ABA响应元件ABRE等,构建了LeNCED1基因上游调控序列与报告基因GUS融合的植物双元表达载体pLeNCED1p:GUS。
The cloning of 1456-bp 5′-flanking sequence of LeNCED 1 gene from tomato and its fusion with β-glucuronidase gene （GUS） were reported. The flanking sequence was bioinformatically analyzed in the database of plant cis-acting regulatory element （PlantCARE）. The result showed that there were several important cis-acting elements, including Circadian （response to circadian rhythm）, Box I （response to light）, TC-rich repeats （response to stress） and ABRE （response to ABA）, in the upstream regulatory region of LeNCED1 gene. The binary vector harboring 5′-flanking sequence of LeNCED1 gene fused with GUS gene originated from pCAMBIA1301 was constructed to be used in coming plant transgene.
出处
《核农学报》
CAS
CSCD
北大核心
2007年第3期232-236,共5页
Journal of Nuclear Agricultural Sciences
基金
广东省自然科学基金博士科研启动基金(06301202)
仲恺农业技术学院博士启动基金(G2360225)
广东省自然科学基金(06025049)
关键词
番茄
NCED基因
5′-侧翼序列克隆
融合基因构建
tomato
9-cis-epoxycarotenoid dioxygenase （NCED） gene
5′-flanking sequence cloning
fusion gene construction
