摘要
采用Lipofectin阳离子脂质体介导质粒DNA转染藏鸡成纤维细胞，通过优化重组质粒转染细胞的各种参数以寻求最佳的转染条件。结果表明：6孔培养板中细胞汇合度迭70％～80％时，用2μL脂质体介导1．5μg重组质粒转染10h即可获得较满意的转染效率。说明Lipofectin能有效介导质粒pEGFP-N3转染藏鸡成纤维细胞，转染率与细胞生长汇合程度、脂质体包被质粒的浓度比例及转染时间直接相关。
In transfecting Zang chicken＇s fibroblast with lipofectin liposome mediated plasmid DNA, the optimum transfection condition was explored through combinating all kinds of index of recombinant plasmid transfected cells. The resuits indicated that the highest efficiency was achieved with cultured fibroblast at 70% -80 % confluences. The essential transfection conditions for these efficiencies were in coating pEGFP-N3 of 1.5μg DNA /100 μL with lipofectin 2 μL/100 μL, and exposing the fibroblast to the DNA-lipofectin complexes for 48 hours. This results indicated that lipofectin could effectively transfer expression pEGFP-N3 plasmid into Zang chicken＇s fibroblast, DNA/lipofectin mixing concentration ratio, and exposure time of fibroblast to DNA lipofectin complexes.
出处
《山西农业大学学报（自然科学版）》
CAS
2007年第2期175-177,共3页
Journal of Shanxi Agricultural University(Natural Science Edition)
