摘要
背景:骨髓间充质细胞具多向分化潜能,可分化为自律性跳动的心肌细胞,而经静脉移植和骨髓动员骨髓间充质细胞治疗扩张型心肌病心力衰竭的报道较少。目的:观察骨髓间充质细胞静脉移植联合自体骨髓干细胞动员对心肌病心力衰竭大鼠心功能的影响。设计、时间及地点:对照观察实验,于2007-01-10/2008-01-10在山西医科大学国家重点生理实验室、山西医科大学分子实验室、山西医科大学实验动物中心、山西医科大学中心实验室完成。材料:Wistar大鼠5只作为骨髓间充质细胞供体。50只以阿霉素腹腔注射6周,15mg/kg制作大鼠心肌病心力衰竭模型。重组人粒细胞刺激因子购于齐鲁制药有限公司。CD44、CD34、CD45均为山西医科大学实验中心流式细胞仪实验室提供。方法:分离纯化大鼠骨髓间充质细胞进行体外培养,采用流式细胞仪检测其表面标记CD45、CD44和CD34。造模后随机分成4组:联合治疗组尾静脉注射骨髓间充质细胞和皮下注入重组人粒细胞刺激因子50μg/kg,自体骨髓干细胞动员组皮下注入重组人粒细胞刺激因子50μg/kg,骨髓间充质干细胞组为尾静脉注射骨髓间充质细胞,心力衰竭组不给予任何干预。主要观察指标:①治疗前及治疗后第6天大鼠外周血白细胞计数和单个核细胞数及CD34表达。②造模4周后以彩色超声心动图和血液动力学检查评价各组大鼠心功能参数。结果:与治疗前相比,联合治疗组、自体骨髓干细胞动员组治疗后第6天外周血白细胞计数和单个核细胞数明显增高(P<0.05),心力衰竭组则无明显变化。联合治疗组、自体骨髓干细胞动员组CD34阳性,心力衰竭组CD34阴性。联合治疗组、自体骨髓干细胞动员组及骨髓间充质细胞组造模4周后血液动力学指标及心脏超声结果显示扩张型心肌病心力衰竭模型大鼠心功能得到明显改善,以联合治疗组改善效果更好。�
BACKGROUND： Bone marrow mesenchymal stem cells （BMSCs） possess the potential in multi-directional differentiation and can differentiate into cardiomyocytes, which had self-discipline jumpiness. There are few reports on intravenous transplantation or autologous mobilization of BMSCs for treating dilated cardiomyopathy-induced heart failure.
OBJECTIVE： To study the effect of BMSC intravenous transplantation combined with bone marrow stem cell autologous mobilization on cardiac function in rats with cardiomyopathy-induced heart failure.
DESIGN, TIME AND SETTING： This control experiment was conducted at the National Physiological Laboratory, Molecule Laboratory, Animal Experimental Center and Central Laboratory of Shanxi Medical University from January 10^th, 2007 to January 10^th, 2008.
MATERIALS： Five Wistar rats served as BMSC donor. Fifty rat models of cardiomyopathy-induced heart failure were created by intraperitoneally injected with 15 mg/kg adriamycin for six weeks. Recombinant human granulocyte stimulating factor （GSF） was bought from Qilu Pharmacy Co., Ltd. CD44, CD34 and CIM5 were given by Flow Cytometric Analysis Laboratory, Experimental Center, Sbanxi Medical University.
METHODS： BMSCs were isolated, purified and cultured in vitro. Surface marker CD45, CDD44 and CD34 were detected with flow cytometer. Wistar rat models were randomized into 4 groups. BMSCs and recombinant human GSF （50 μ g/kg） were injected into models by tail vein and hypodermoclysis infusion respectively in the combination treatment group （MG group）. Recombinant human GSF （50 μ g/kg） was subcutaneously injected into models in the bone marrow stem cell autologous mobilization grouP （G group）. BMSCs were injected into rat models by tail vein in the BMSC group. Rat models in the heart failure group were intact.
MAIN OUTCOME MEASURES： （1）Peripheral blood leukocyte and mononuclear cell counts and CD34 expression were obtained before treatment and 6 days after treatment. （2）At 4 weeks, c
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第29期5645-5648,共4页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
山西省卫生厅科技攻关计划项目(200738)课题名称:骨髓干细胞动员对心肌病心力衰竭大鼠方面研究
山西医科大学博士基金~~
